Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LLG50_RS05185 Genome accession   NZ_CP025500
Coordinates   1049036..1050358 (-) Length   440 a.a.
NCBI ID   WP_103054704.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain G50     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1044036..1055358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLG50_RS05155 (LLG50_05155) - 1045179..1046147 (+) 969 WP_003130649.1 PhoH family protein -
  LLG50_RS05160 (LLG50_05160) - 1046205..1046684 (+) 480 WP_021722517.1 NUDIX hydrolase -
  LLG50_RS05165 (LLG50_05165) ybeY 1046836..1047324 (+) 489 WP_021722516.1 rRNA maturation RNase YbeY -
  LLG50_RS05170 (LLG50_05170) - 1047308..1047763 (+) 456 WP_003130653.1 diacylglycerol kinase family protein -
  LLG50_RS05175 (LLG50_05175) - 1047777..1048355 (+) 579 WP_103054703.1 nucleotidyltransferase family protein -
  LLG50_RS11805 comFC 1048389..1048793 (-) 405 WP_021722514.1 ComF family protein Machinery gene
  LLG50_RS05185 (LLG50_05185) comFA 1049036..1050358 (-) 1323 WP_103054704.1 DEAD/DEAH box helicase Machinery gene
  LLG50_RS05190 (LLG50_05190) - 1050415..1051044 (+) 630 WP_003130660.1 YigZ family protein -
  LLG50_RS05195 (LLG50_05195) - 1051178..1051675 (+) 498 WP_003130662.1 VanZ family protein -
  LLG50_RS05200 (LLG50_05200) - 1051723..1052742 (+) 1020 WP_012897709.1 DUF475 domain-containing protein -
  LLG50_RS05205 (LLG50_05205) - 1052857..1053603 (+) 747 WP_187398315.1 bifunctional metallophosphatase/5'-nucleotidase -
  LLG50_RS05210 (LLG50_05210) glyQ 1053889..1054842 (+) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50164.65 Da        Isoelectric Point: 10.0552

>NTDB_id=262090 LLG50_RS05185 WP_103054704.1 1049036..1050358(-) (comFA) [Lactococcus lactis subsp. lactis strain G50]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLAGPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFQAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKITSVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=262090 LLG50_RS05185 WP_103054704.1 1049036..1050358(-) (comFA) [Lactococcus lactis subsp. lactis strain G50]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTATTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAACAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTGGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCAAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATCACTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTCTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGGAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(300-403)

Type III restriction enzyme, res subunit (ResIII)

(114-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74

90.909

0.673
  comFA/cflA Streptococcus mitis SK321

51.508

90.455

0.466
  comFA/cflA Streptococcus pneumoniae Rx1

51.508

90.455

0.466
  comFA/cflA Streptococcus pneumoniae D39

51.508

90.455

0.466
  comFA/cflA Streptococcus pneumoniae R6

51.508

90.455

0.466
  comFA/cflA Streptococcus pneumoniae TIGR4

51.508

90.455

0.466
  comFA/cflA Streptococcus mitis NCTC 12261

50.754

90.455

0.459
  comFA Bacillus subtilis subsp. subtilis str. 168

36.807

100

0.377

Multiple sequence alignment