Detailed information NTG0261700

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: CPT07_RS15930
• Genome accession: NZ_CP030919
• Coordinates: 3023959..3024618 (+)
• Length: 219 a.a.
• NCBI ID: WP_001221502.1
• Uniprot ID: B7LGG8
• Organism: Escherichia coli strain KL53
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3018959..3029618

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CPT07_RS15905 (CPT07_15905)	mqsA	3020669..3021064 (-)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
CPT07_RS15910 (CPT07_15910)	mqsR	3021066..3021362 (-)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
CPT07_RS15915 (CPT07_15915)	-	3021567..3021992 (-)	426	WP_044864279.1	GyrI-like domain-containing protein	-
CPT07_RS15920 (CPT07_15920)	-	3022083..3023296 (+)	1214	WP_139371347.1	IS3 family transposase	-
CPT07_RS15925 (CPT07_15925)	ygiW	3023415..3023807 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
CPT07_RS15930 (CPT07_15930)	ciaR	3023959..3024618 (+)	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	Regulator
CPT07_RS15935 (CPT07_15935)	qseC	3024615..3025964 (+)	1350	WP_000673354.1	quorum sensing histidine kinase QseC	-
CPT07_RS15940 (CPT07_15940)	ygiZ	3026010..3026342 (-)	333	WP_000914691.1	DUF2645 family protein	-
CPT07_RS15945 (CPT07_15945)	-	3026349..3027059 (-)	711	WP_000834029.1	hypothetical protein	-
CPT07_RS15950 (CPT07_15950)	-	3027062..3027430 (-)	369	Protein_2940	Hcp family type VI secretion system effector	-
CPT07_RS15965 (CPT07_15965)	-	3028198..3028317 (-)	120	Protein_2942	type VI secretion system tube protein Hcp	-
CPT07_RS15970 (CPT07_15970)	mdaB	3028567..3029148 (+)	582	WP_085381306.1	NADPH:quinone oxidoreductase MdaB	-
CPT07_RS15975 (CPT07_15975)	ygiN	3029179..3029493 (+)	315	WP_000958598.1	putative quinol monooxygenase	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24745.66 Da
• Isoelectric Point: 6.6543

>NTDB_id=261700 CPT07_RS15930 WP_001221502.1 3023959..3024618(+) (ciaR) [Escherichia coli strain KL53]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=261700 CPT07_RS15930 WP_001221502.1 3023959..3024618(+) (ciaR) [Escherichia coli strain KL53]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: B7LGG8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365