Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CWE28_RS20720 Genome accession   NZ_CP030861
Coordinates   4300844..4301515 (+) Length   223 a.a.
NCBI ID   WP_003085524.1    Uniprot ID   A0A0H2Z6L5
Organism   Pseudomonas aeruginosa strain HS9     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4295844..4306515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWE28_RS20700 (CWE28_20695) - 4296290..4297807 (-) 1518 WP_003120784.1 tripartite tricarboxylate transporter permease -
  CWE28_RS20705 (CWE28_20700) - 4297818..4298288 (-) 471 WP_023114050.1 tripartite tricarboxylate transporter TctB family protein -
  CWE28_RS20710 (CWE28_20705) - 4298337..4299320 (-) 984 WP_003085519.1 tripartite tricarboxylate transporter substrate binding protein -
  CWE28_RS20715 (CWE28_20710) - 4299350..4300633 (-) 1284 WP_003116508.1 OprD family porin -
  CWE28_RS20720 (CWE28_20715) ciaR 4300844..4301515 (+) 672 WP_003085524.1 response regulator Regulator
  CWE28_RS20725 (CWE28_20720) - 4301508..4302890 (+) 1383 WP_010793854.1 sensor histidine kinase -
  CWE28_RS20730 (CWE28_20725) - 4302899..4303735 (-) 837 WP_003085528.1 HDOD domain-containing protein -
  CWE28_RS20735 (CWE28_20730) - 4303836..4304780 (-) 945 WP_003101947.1 folate-binding protein YgfZ -
  CWE28_RS20740 (CWE28_20735) - 4305053..4305307 (+) 255 WP_003085532.1 succinate dehydrogenase assembly factor 2 -
  CWE28_RS20745 (CWE28_20740) - 4305291..4305743 (+) 453 WP_003114181.1 protein YgfX -

Sequence

Protein


Download         Length: 223 a.a.        Molecular weight: 24431.24 Da        Isoelectric Point: 5.1881

>NTDB_id=261122 CWE28_RS20720 WP_003085524.1 4300844..4301515(+) (ciaR) [Pseudomonas aeruginosa strain HS9]
MRILLVEDHPQLAESVVQALKGAGWTVDLLQDGVAADLALASEEYALAILDVGLPRMDGFEVLARLRGRGKTLPVLMLTA
RGEVKDRVHGLNLGADDYLAKPFELSELEARVKALLRRSVLGGEQLQRCGALVYDLGTRRFSLDEQPLTLTSREQAVLEA
MIARPGRVMSKEQLAAQVFGLDEEASADAIEIYVHRLRKKLEGGAVRIVTFRGLGYLLEAQGD

Nucleotide


Download         Length: 672 bp        

>NTDB_id=261122 CWE28_RS20720 WP_003085524.1 4300844..4301515(+) (ciaR) [Pseudomonas aeruginosa strain HS9]
ATGCGCATCCTTCTGGTGGAAGATCATCCGCAGCTCGCCGAAAGCGTGGTCCAGGCGCTCAAGGGCGCCGGCTGGACGGT
AGACCTGCTGCAGGACGGGGTGGCCGCCGACCTGGCCCTGGCCAGCGAGGAGTACGCCCTGGCGATCCTCGACGTCGGCC
TGCCGCGGATGGACGGCTTCGAGGTGCTGGCGCGCCTGCGCGGGCGCGGCAAGACCCTGCCGGTGCTGATGCTGACTGCG
CGCGGCGAGGTGAAGGACCGGGTGCACGGCCTCAATCTCGGCGCCGACGACTACCTGGCAAAGCCTTTCGAACTGTCCGA
GCTGGAGGCGCGGGTCAAGGCCCTGCTGCGCCGCAGCGTGCTCGGTGGCGAACAGTTGCAGCGCTGCGGCGCCCTGGTCT
ACGACCTCGGCACCCGGCGCTTCAGCCTCGACGAGCAGCCGCTGACCCTGACCTCGCGGGAACAGGCGGTGCTGGAAGCG
ATGATCGCCCGTCCGGGGCGGGTGATGAGCAAGGAGCAACTGGCCGCCCAGGTCTTCGGCCTGGACGAGGAGGCCAGCGC
CGACGCCATCGAGATCTACGTCCACCGCCTGCGCAAGAAGCTGGAAGGCGGCGCCGTGCGCATCGTCACCTTCCGCGGCC
TCGGCTACCTGCTGGAGGCGCAGGGTGACTGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(146-218)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z6L5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.668

100

0.377
  ciaR Streptococcus pneumoniae D39

37.668

100

0.377
  ciaR Streptococcus pneumoniae R6

37.668

100

0.377
  ciaR Streptococcus pneumoniae TIGR4

37.668

100

0.377
  covR Lactococcus lactis subsp. lactis strain DGCC12653

37.668

100

0.377

Multiple sequence alignment