Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   DSB71_RS05490 Genome accession   NZ_CP030768
Coordinates   1095913..1096572 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain 2017C-4173W12     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1090913..1101572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSB71_RS05460 ygiS 1092196..1093803 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  DSB71_RS05470 mqsA 1093936..1094331 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  DSB71_RS05475 mqsR 1094333..1094629 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  DSB71_RS05480 ygiV 1094834..1095316 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  DSB71_RS05485 ygiW 1095369..1095761 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  DSB71_RS05490 ciaR 1095913..1096572 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  DSB71_RS05495 qseC 1096569..1097918 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  DSB71_RS05500 ygiZ 1097964..1098296 (-) 333 WP_000917684.1 DUF2645 family protein -
  DSB71_RS05510 mdaB 1098624..1099205 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  DSB71_RS05515 ygiN 1099236..1099550 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  DSB71_RS05520 parE 1099598..1101490 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=260366 DSB71_RS05490 WP_001221493.1 1095913..1096572(+) (ciaR) [Escherichia coli strain 2017C-4173W12]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=260366 DSB71_RS05490 WP_001221493.1 1095913..1096572(+) (ciaR) [Escherichia coli strain 2017C-4173W12]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365