Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   DSB73_RS19290 Genome accession   NZ_CP030767
Coordinates   3488203..3488862 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2017C-4109     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3483203..3493862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSB73_RS19275 ygiS 3485408..3487015 (-) 1608 WP_001301989.1 ABC transporter substrate-binding protein -
  DSB73_RS19280 ygiV 3487124..3487606 (-) 483 WP_000183479.1 GyrI-like domain-containing protein -
  DSB73_RS19285 ygiW 3487659..3488051 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  DSB73_RS19290 ciaR 3488203..3488862 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  DSB73_RS19295 qseC 3488859..3490208 (+) 1350 WP_000673362.1 quorum sensing histidine kinase QseC -
  DSB73_RS19300 mdaB 3490318..3490899 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  DSB73_RS19305 ygiN 3490930..3491244 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  DSB73_RS19310 - 3491289..3492176 (-) 888 WP_000614950.1 MurR/RpiR family transcriptional regulator -
  DSB73_RS19315 - 3492173..3493120 (-) 948 WP_001301797.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=260308 DSB73_RS19290 WP_001221502.1 3488203..3488862(+) (ciaR) [Escherichia coli strain 2017C-4109]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=260308 DSB73_RS19290 WP_001221502.1 3488203..3488862(+) (ciaR) [Escherichia coli strain 2017C-4109]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment