Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CXR48_RS12300 Genome accession   NZ_CP025341
Coordinates   2556925..2557539 (-) Length   204 a.a.
NCBI ID   WP_015387983.1    Uniprot ID   A0A6A8LHQ3
Organism   Bacillus velezensis strain CMT-6     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2551925..2562539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXR48_RS12275 rpsT 2552225..2552491 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  CXR48_RS12280 holA 2552508..2553549 (-) 1042 Protein_2373 DNA polymerase III subunit delta -
  CXR48_RS19500 - 2553589..2553743 (-) 155 Protein_2374 hypothetical protein -
  CXR48_RS12285 - 2553784..2553918 (+) 135 WP_003152870.1 YqzM family protein -
  CXR48_RS12290 comEC 2553937..2556288 (-) 2352 WP_063174744.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  CXR48_RS12295 - 2556289..2556858 (-) 570 WP_003152868.1 ComE operon protein 2 -
  CXR48_RS12300 comEA 2556925..2557539 (-) 615 WP_015387983.1 helix-hairpin-helix domain-containing protein Machinery gene
  CXR48_RS12305 comER 2557598..2558419 (+) 822 WP_014305449.1 late competence protein ComER -
  CXR48_RS12310 - 2558488..2559225 (-) 738 WP_014305450.1 class I SAM-dependent DNA methyltransferase -
  CXR48_RS12315 rsfS 2559222..2559578 (-) 357 WP_003152864.1 ribosome silencing factor -
  CXR48_RS12320 yqeK 2559575..2560156 (-) 582 WP_063174743.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  CXR48_RS12325 - 2560146..2560715 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  CXR48_RS12330 yhbY 2560726..2561016 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  CXR48_RS12335 aroE 2561010..2561852 (-) 843 WP_025852995.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22255.41 Da        Isoelectric Point: 5.5211

>NTDB_id=260181 CXR48_RS12300 WP_015387983.1 2556925..2557539(-) (comEA) [Bacillus velezensis strain CMT-6]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=260181 CXR48_RS12300 WP_015387983.1 2556925..2557539(-) (comEA) [Bacillus velezensis strain CMT-6]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGTGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCTATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LHQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment