Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CV094_RS08445 Genome accession   NZ_CP025266
Coordinates   1764653..1765393 (-) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii isolate SMC_Paed_Ab_BL01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1759653..1770393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CV094_RS08425 (CV094_08425) aroB 1760193..1761275 (-) 1083 WP_001193997.1 3-dehydroquinate synthase -
  CV094_RS08430 (CV094_08430) aroK 1761304..1761873 (-) 570 WP_000643712.1 shikimate kinase AroK -
  CV094_RS08435 (CV094_08435) pilQ 1761885..1764047 (-) 2163 WP_033503048.1 type IV pilus secretin PilQ family protein Machinery gene
  CV094_RS08440 (CV094_08440) pilP 1764115..1764642 (-) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  CV094_RS08445 (CV094_08445) pilO 1764653..1765393 (-) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  CV094_RS08450 (CV094_08450) pilN 1765390..1766031 (-) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  CV094_RS08455 (CV094_08455) pilM 1766031..1767089 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  CV094_RS08460 (CV094_08460) ponA 1767252..1769807 (+) 2556 WP_000736677.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=259666 CV094_RS08445 WP_000076101.1 1764653..1765393(-) (pilO) [Acinetobacter baumannii isolate SMC_Paed_Ab_BL01]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=259666 CV094_RS08445 WP_000076101.1 1764653..1765393(-) (pilO) [Acinetobacter baumannii isolate SMC_Paed_Ab_BL01]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGCATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment