Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexA   Type   Machinery gene
Locus tag   DOU34_RS07535 Genome accession   NZ_CP030097
Coordinates   1479244..1481829 (+) Length   861 a.a.
NCBI ID   WP_007409829.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SH-B74     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1474244..1486829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU34_RS07515 - 1475043..1476221 (+) 1179 WP_113629803.1 glycine C-acetyltransferase -
  DOU34_RS07520 miaB 1476364..1477893 (+) 1530 WP_012117585.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  DOU34_RS07525 ymcA 1477896..1478327 (+) 432 WP_003154113.1 RicAFT regulatory complex protein RicA family protein Regulator
  DOU34_RS07530 cotE 1478580..1479125 (+) 546 WP_003154111.1 outer spore coat protein CotE -
  DOU34_RS07535 hexA 1479244..1481829 (+) 2586 WP_007409829.1 DNA mismatch repair protein MutS Machinery gene
  DOU34_RS07540 hexB 1481845..1483722 (+) 1878 WP_007409830.1 DNA mismatch repair endonuclease MutL Machinery gene
  DOU34_RS07545 - 1483774..1484388 (-) 615 WP_020955840.1 site-specific integrase -
  DOU34_RS07550 - 1484826..1485128 (+) 303 WP_007409833.1 hypothetical protein -
  DOU34_RS07560 - 1486354..1486563 (-) 210 WP_020955841.1 hypothetical protein -

Sequence

Protein


Download         Length: 861 a.a.        Molecular weight: 97737.93 Da        Isoelectric Point: 5.3045

>NTDB_id=258550 DOU34_RS07535 WP_007409829.1 1479244..1481829(+) (hexA) [Bacillus amyloliquefaciens strain SH-B74]
MASYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGSADKIPMCGVPYHSAAAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSEFRDGYGLALSDLTTGENLAVFIERIEDV
MSEIYSVGAKEIVVSSKFNEHTAAQLKERCGATISIEDGEITERIEIAKHLPGEELTETFMRLYTYLQKTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKSKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRASQIEERQEMVETLINHLF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPSIKELVGSLNHKKAKERAGLIDPCGDLLDLLEEALHE
NPPLSLKEGNLIKDGYHQKLDEYRDASKNGKDWIARLEQQERAYTGIRSLKVGFNKVFGYYIEVTKANLHLLEDGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFAGLREKVKQFIPRLQRLAKQMSELDALQCFATISENRHYTKPVF
SDNEVKVIEGRHPVVEKVMDSQEYVPNNCLMGDSREMLLITGPNMSGKSTYMRQIALLSIMAQIGCFVPAKEAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKDSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPDDLISRAQEILKQLEQTGDKPELPAVSEKK
SAVREEPAQLSFFADGEKEQKAPAVSNKEKQVLEAFKSINILDMTPLEAMNEMYKLQKKLK

Nucleotide


Download         Length: 2586 bp        

>NTDB_id=258550 DOU34_RS07535 WP_007409829.1 1479244..1481829(+) (hexA) [Bacillus amyloliquefaciens strain SH-B74]
ATGGCTAGTTACACGCCTATGATACAGCAATACTTGAAGATAAAGGCAGAGCACCAAGATGCCTTTTTATTTTTTCGGCT
TGGTGACTTTTATGAAATGTTTTTTGAAGATGCAAAAAAAGCGTCACAAGAGCTTGAAATTACACTGACAAGCAGAGACG
GAGGTTCGGCTGATAAAATTCCGATGTGCGGCGTGCCTTATCATTCCGCGGCGGCTTATATTGAACAGCTGATTAAAAAG
GGCTATAAAGTGGCGATCTGTGAACAGACGGAAGATCCGAAGGCGGCTAAAGGCGTTGTAAAAAGAGAAGTCGTCCAGCT
GATCACACCGGGAACCGTGATGGATGGAAAAGGCATCCACGAGTCGGAAAACAACTTTATCGCTTCCGTTTCCGAATTTC
GTGACGGATACGGTCTCGCTTTATCGGACTTAACGACCGGAGAAAACCTGGCCGTATTTATTGAGCGGATTGAAGATGTC
ATGTCAGAAATTTACTCAGTCGGCGCAAAGGAAATTGTCGTATCGAGCAAGTTTAACGAACATACAGCTGCTCAGCTGAA
AGAACGGTGCGGCGCCACGATTTCCATCGAAGACGGGGAAATAACAGAACGCATTGAAATCGCAAAGCACTTGCCGGGCG
AAGAGCTGACAGAGACGTTTATGCGTTTATATACGTATTTGCAGAAAACGCAAAAACGGAGCCTGGATCACCTGCAGCCG
GTTCAAGTATATGAATTGGAAGAAGCGATGAAAATTGACCTGTATTCAAAACGGAATCTTGAGCTGACGGAAACGATCCG
TTCGAAAAGCAAAAAAGGGTCATTGCTTTGGCTTTTAGACGAGACGAAAACAGCGATGGGCGGCCGGCTTCTCAAACAAT
GGATTGACCGCCCGCTGATCAGGGCATCCCAAATCGAAGAACGCCAGGAAATGGTCGAGACGCTGATCAATCATCTGTTT
GAGCGTGAAGATCTGCGAGAGCGCCTGAAGGAAGTATACGATTTAGAACGTCTGGCGGGCCGGGTCGCATTCGGAAACGT
CAACGCAAGGGATTTAATCCAGCTGAAAGAATCTTTAAAACAAGTGCCGAGCATCAAAGAGCTTGTAGGGTCTCTTAACC
ATAAAAAAGCAAAAGAACGCGCCGGCTTGATTGATCCGTGCGGAGATTTGCTTGACCTGTTAGAAGAAGCCCTTCATGAA
AACCCGCCGTTATCGTTAAAAGAAGGCAATTTAATTAAAGACGGCTATCATCAGAAGCTCGATGAGTACCGTGACGCCAG
CAAAAACGGTAAAGACTGGATTGCCAGACTGGAGCAGCAGGAACGCGCTTATACGGGCATCCGTTCATTAAAGGTCGGTT
TCAATAAAGTGTTCGGCTATTATATTGAAGTCACAAAAGCAAACCTTCATCTGCTTGAAGACGGACGGTATGAGCGGAAA
CAGACCCTCACAAACGCCGAACGGTACATTACGCCGGAATTGAAGGAAAAAGAAGCGCTTATTTTAGAAGCGGAAAACAA
CATCTGCGAGCTGGAATATGAGCTTTTTGCCGGACTGCGTGAAAAGGTGAAACAATTTATTCCGCGCTTGCAGCGTCTTG
CCAAACAAATGAGTGAGCTGGACGCACTGCAATGCTTTGCTACGATCAGTGAAAACAGGCATTACACAAAGCCCGTCTTT
TCTGATAATGAAGTAAAAGTCATTGAAGGGAGACACCCCGTCGTTGAAAAGGTGATGGACAGTCAAGAATACGTCCCGAA
CAACTGCCTGATGGGCGATTCGCGGGAAATGCTCCTGATTACGGGTCCGAATATGTCAGGTAAAAGCACGTACATGAGAC
AGATCGCACTTCTATCGATCATGGCCCAGATCGGCTGTTTTGTGCCCGCGAAAGAAGCGGTTCTGCCGATTTTTGATCAA
ATCTTCACGAGAATCGGGGCAGCGGATGATTTGATTTCCGGACAAAGCACCTTTATGGTGGAAATGCTGGAAGCGAAAAA
CGCGATTGTGAACGCCACAAAAGATAGCCTGATCCTGTTTGATGAAATCGGCCGCGGGACATCAACGTACGACGGAATGG
CGCTGGCACAGGCGATTATTGAATATGTCCATGATCATATCGGCGCTAAAACACTGTTCAGCACGCATTATCATGAGCTG
ACGGTGCTTGAAGATAAGCTTCCTCAGCTGAAAAACGTTCATGTGCGCGCAGAGGAATATAACGGAACGGTCGTGTTTCT
CCATCAAATCAAAGAAGGAGCCGCAGATAAAAGTTACGGCATTCATGTCGCCCAGCTTGCCGAGCTCCCGGACGATCTGA
TCAGCCGCGCGCAGGAGATTTTGAAACAGCTTGAGCAGACGGGAGATAAACCGGAGCTTCCGGCGGTATCTGAGAAAAAG
TCCGCCGTCAGAGAAGAACCGGCACAGCTGTCCTTTTTTGCTGACGGTGAAAAAGAACAGAAGGCACCGGCTGTTTCTAA
TAAAGAAAAACAAGTGCTTGAAGCTTTTAAGTCAATCAATATTCTCGATATGACGCCGCTTGAGGCGATGAATGAAATGT
ACAAGCTGCAAAAAAAATTAAAATAG

Domains

Predicted by InterProScan.

MutS family domain IV (MutS_IV)

(415-506)

MutS domain II (MutS_II)

(125-239)

MutS domain V (MutS_V)

(598-785)

MutS domain I (MutS_I)

(5-116)

MutS domain III (MutS_III)

(258-547)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexA Streptococcus pneumoniae R6

48.191

99.535

0.48
  mutS Pseudomonas stutzeri strain ATCC 17587

38.435

100

0.394