Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   CWI35_RS16260 Genome accession   NZ_CP025074
Coordinates   3129703..3129999 (+) Length   98 a.a.
NCBI ID   WP_011231915.1    Uniprot ID   U2X350
Organism   [Bacillus] caldolyticus strain NEB414     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 3124703..3134999
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI35_RS16235 (CWI35_16205) - 3124893..3126026 (+) 1134 WP_119878023.1 SAM-dependent methyltransferase -
  CWI35_RS16240 (CWI35_16210) - 3126110..3126352 (-) 243 WP_008879854.1 DUF2626 family protein -
  CWI35_RS16245 (CWI35_16215) - 3126511..3127209 (-) 699 WP_011231918.1 helix-turn-helix domain-containing protein -
  CWI35_RS16250 (CWI35_16220) comGA 3127402..3128475 (+) 1074 WP_021322348.1 competence type IV pilus ATPase ComGA Machinery gene
  CWI35_RS16255 (CWI35_16225) comGB 3128472..3129500 (+) 1029 WP_047758066.1 competence type IV pilus assembly protein ComGB -
  CWI35_RS16260 (CWI35_16230) comGC 3129703..3129999 (+) 297 WP_011231915.1 competence type IV pilus major pilin ComGC Machinery gene
  CWI35_RS16265 (CWI35_16235) comGD 3129989..3130426 (+) 438 WP_011231914.1 competence type IV pilus minor pilin ComGD -
  CWI35_RS16270 (CWI35_16240) - 3130410..3130736 (+) 327 WP_047758065.1 type II secretion system protein -
  CWI35_RS16275 (CWI35_16245) comGF 3130715..3131161 (+) 447 WP_047758064.1 competence type IV pilus minor pilin ComGF -
  CWI35_RS16280 (CWI35_16250) comGG 3131236..3131625 (+) 390 WP_047758063.1 competence type IV pilus minor pilin ComGG -
  CWI35_RS16285 (CWI35_16255) - 3131672..3131851 (+) 180 WP_011231910.1 YqzE family protein -
  CWI35_RS16290 (CWI35_16260) - 3131954..3132748 (-) 795 WP_119877923.1 YqhG family protein -
  CWI35_RS16295 (CWI35_16265) - 3132735..3134399 (-) 1665 WP_119877924.1 SNF2-related protein -

Sequence


Protein


Download         Length: 98 a.a.        Molecular weight: 10749.59 Da        Isoelectric Point: 7.2422

>NTDB_id=258050 CWI35_RS16260 WP_011231915.1 3129703..3129999(+) (comGC) [[Bacillus] caldolyticus strain NEB414]
MNQKGFTLIEMLIVMMVISVLLLIAIPNMTKHNSMINSKGCEAFLNTVQAQVKAYEMEHNKIPTVQELLAGRYIKSDKCP
NGHAIQISANGDVSESGS

Nucleotide


Download         Length: 297 bp        

>NTDB_id=258050 CWI35_RS16260 WP_011231915.1 3129703..3129999(+) (comGC) [[Bacillus] caldolyticus strain NEB414]
ATGAATCAAAAGGGATTCACATTGATCGAAATGTTGATCGTTATGATGGTGATTTCTGTCCTGCTGCTGATTGCCATTCC
GAATATGACAAAGCACAACAGCATGATCAATTCGAAAGGATGCGAGGCGTTTTTGAACACCGTGCAAGCGCAGGTGAAAG
CGTATGAGATGGAGCATAACAAAATTCCGACGGTGCAGGAATTGCTCGCTGGCCGCTATATCAAGTCGGACAAATGTCCG
AACGGTCATGCGATCCAAATCAGCGCGAACGGCGATGTGAGTGAAAGTGGCTCGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB U2X350

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

52.747

92.857

0.49

  comGC Staphylococcus aureus MW2

41.935

94.898

0.398

  comGC Staphylococcus aureus N315

41.935

94.898

0.398


Multiple sequence alignment