Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   CWI35_RS06765 Genome accession   NZ_CP025074
Coordinates   1291873..1295601 (-) Length   1242 a.a.
NCBI ID   WP_119877622.1    Uniprot ID   -
Organism   [Bacillus] caldolyticus strain NEB414     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1286873..1300601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI35_RS06750 (CWI35_06740) - 1286879..1287100 (+) 222 WP_011230188.1 spore germination protein -
  CWI35_RS06755 (CWI35_06745) - 1287333..1290677 (-) 3345 WP_119877621.1 SMC family ATPase -
  CWI35_RS06760 (CWI35_06750) - 1290674..1291852 (-) 1179 WP_021322517.1 exonuclease SbcCD subunit D -
  CWI35_RS06765 (CWI35_06755) addA 1291873..1295601 (-) 3729 WP_119877622.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  CWI35_RS06770 (CWI35_06760) addB 1295602..1299105 (-) 3504 WP_286365221.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  CWI35_RS06775 (CWI35_06765) lepB 1299329..1299877 (-) 549 WP_011230183.1 signal peptidase I -
  CWI35_RS06780 (CWI35_06770) - 1299874..1300497 (-) 624 WP_013146210.1 TVP38/TMEM64 family protein -

Sequence

Protein


Download         Length: 1242 a.a.        Molecular weight: 141385.69 Da        Isoelectric Point: 5.8448

>NTDB_id=258019 CWI35_RS06765 WP_119877622.1 1291873..1295601(-) (addA) [[Bacillus] caldolyticus strain NEB414]
MNATIRPKPAGSRWTDEQWKAIAAGGRDILVAAAAGSGKTAVLVERIIQKVTAEEGAVDIDRLLVVTFTNAAAAEMKARI
GEALERELAKRPHSLHLRRQLSLLPRAAISTLHSFCLDVIRKYYYLLDLDPSFRIADETEIELLKEDVLEELLEEEYGKP
DNERFFAVVDAYTGDRSDAELQEMIVALYEFSRSHPEPDEWLAGLTSMYDVDERTDIKTLPAARYIAQHAAMELAAARRL
IRRALELAEEPGGPRPYAERLREDRDMIADLETRLSGPWAELHRALKALSFGRLPACRGKDYDERLIDEAKSLRDQAKKK
VEALRDNVFSLDPSVWLRHMREMKPIVETIANLVRRFAVMFQAVKREKGIVDFSDLEHYCLHILRRRDPETGEWQPSPAA
LEYQAQFDEVLVDEYQDTNLVQEAILQLVKKGSERTGNLFMVGDVKQSIYRFRLAEPMLFLDKYKRFTADGEEGGMKIDL
ASNFRSRREVLDGTNFLFAQLMGETVGEMVYDEAAQLKYGADYPEGEDAAPEVMIINRQRAAEEDEEEAAEWEAAELEAR
LMAKKIKEIVSAPFYVYDRPSGQPRRAMYRDIVILVRSMTHAPQMIEQLQAQGIPAAADLSSGYFQATEISIMLSLLKVI
DNPYQDIPLAAVLRSPLFRFDENELAMIRLADPKGTFYEALCSFRQKPAETEEEANAQRKAAAFLERLEGWRTMARRRSL
ADLIWLLYRDTQFYDFVGALPGGRQRQANLRALYDRARQYESTSFRGLFRFLRFIERLQERGDDLGAARPLGEQEDVVRI
MTIHSSKGLEFPIVFLAGLARSFHTRDLHHPYLLDKELGFAARFVHPRLRISYPTLPLLAIQTKKRLELLAEEMRILYVA
LTRAKEKLYLLASVNDAAKEIEKWKSAASERGWLLPDDVRASARSYLDWIGRALIRHRDGGALAGMKAPEEVASHPSVWR
VSIVPAADLRGAEAAREEMDGGVLLALEQGRPVPVEGGWRKEVKRRLLWRYSYEKETAVRAKQSVSELKEHRVLFGEQAD
EWRPRQGTAPVFSRPRFMQEKTLTPAEKGTALHIVMRHLDLQAPLDESSIRSQIVRLVEKELLSAEQAEVVDPAAIAAFF
TADLGRRLCAAREVHREVPFSLGLKAAELYGGEGTESGRRVLVQGVIDCVFVDECGYVMIDYKTDEVVHRFAGQKEEAAR
FLLGRYGTQMRLYRRAIEQIWRAPVAECYLYSFDGGFVVAVE

Nucleotide


Download         Length: 3729 bp        

>NTDB_id=258019 CWI35_RS06765 WP_119877622.1 1291873..1295601(-) (addA) [[Bacillus] caldolyticus strain NEB414]
ATGAACGCCACGATTCGCCCGAAGCCGGCTGGGAGCCGATGGACGGATGAACAATGGAAGGCGATCGCCGCGGGCGGCCG
AGACATTCTTGTCGCCGCGGCGGCTGGATCCGGGAAAACGGCCGTTCTTGTCGAGCGGATCATTCAAAAAGTGACGGCGG
AAGAAGGAGCGGTCGATATCGACCGTCTGCTTGTCGTTACCTTTACCAATGCGGCAGCCGCGGAGATGAAAGCAAGAATC
GGCGAGGCGCTCGAGCGGGAATTGGCCAAGCGTCCGCATTCGCTCCATTTGCGACGTCAACTCAGCCTGCTTCCCCGCGC
CGCGATCTCGACGCTCCATTCCTTTTGCTTGGATGTCATTCGCAAATATTACTATTTGCTTGATTTGGATCCGTCGTTTC
GCATCGCGGATGAAACGGAAATCGAGCTGTTGAAAGAGGATGTCCTTGAAGAGCTGCTTGAGGAGGAATATGGAAAGCCG
GACAACGAGCGGTTTTTCGCTGTCGTGGATGCCTATACGGGCGACCGGAGCGATGCGGAGCTGCAGGAGATGATTGTCGC
CCTTTATGAATTTTCCCGCTCCCATCCGGAACCAGATGAATGGTTGGCCGGTTTGACCTCGATGTATGATGTCGATGAAC
GAACAGATATAAAGACGCTGCCAGCAGCCCGCTATATCGCGCAACATGCTGCGATGGAGCTGGCGGCGGCCAGACGGCTG
ATCCGAAGGGCGCTTGAGCTTGCTGAAGAGCCGGGCGGACCGCGTCCGTACGCGGAGCGGCTGCGCGAAGATCGAGATAT
GATCGCTGATTTGGAAACGCGTCTTTCTGGACCGTGGGCCGAGCTGCATCGCGCGCTAAAGGCGCTGTCGTTTGGGCGGC
TGCCGGCCTGCCGCGGCAAGGACTATGACGAGCGGCTGATCGACGAAGCCAAATCGCTGCGTGATCAGGCGAAAAAGAAA
GTGGAGGCGCTGCGCGACAATGTGTTTTCGCTCGATCCATCCGTTTGGCTCCGCCATATGCGGGAAATGAAGCCGATCGT
TGAAACGATCGCTAACCTTGTCCGCCGTTTTGCCGTCATGTTCCAAGCGGTGAAACGGGAAAAAGGAATCGTTGATTTTT
CTGACTTGGAACATTATTGTTTGCATATTTTGCGGCGGCGCGATCCGGAAACGGGCGAATGGCAGCCTTCCCCCGCCGCC
TTGGAATACCAGGCGCAGTTTGATGAAGTGCTTGTTGACGAGTATCAAGATACGAACCTCGTTCAAGAAGCGATTTTGCA
GCTGGTGAAAAAAGGGAGCGAACGAACAGGCAATTTGTTTATGGTCGGCGACGTGAAACAGTCGATTTACCGATTCCGCC
TTGCCGAGCCGATGCTGTTTTTGGACAAATACAAACGATTCACCGCAGACGGGGAAGAAGGGGGGATGAAAATCGATCTT
GCGAGCAACTTCCGCAGCCGCCGCGAAGTGCTTGACGGGACGAATTTTCTGTTTGCGCAACTCATGGGGGAAACCGTCGG
CGAAATGGTTTACGACGAAGCGGCGCAGTTGAAATACGGCGCTGATTACCCGGAAGGGGAGGATGCGGCGCCGGAAGTCA
TGATCATCAACCGGCAGCGTGCGGCGGAAGAGGACGAAGAGGAAGCGGCTGAATGGGAAGCGGCGGAGCTCGAGGCGCGG
CTGATGGCGAAAAAAATCAAAGAGATCGTTTCCGCTCCGTTTTATGTATACGATCGTCCGAGCGGGCAGCCGCGGCGCGC
CATGTACCGTGACATCGTCATCCTTGTCCGCTCGATGACGCATGCGCCGCAGATGATCGAGCAGCTGCAGGCGCAAGGCA
TCCCGGCGGCCGCGGATTTGTCATCCGGCTATTTTCAGGCGACGGAAATTTCGATCATGCTCTCACTGTTAAAAGTGATC
GACAACCCGTACCAAGACATTCCGCTCGCCGCGGTGCTCCGGTCGCCGCTCTTTCGTTTTGACGAGAATGAGCTCGCCAT
GATTCGCCTCGCCGATCCAAAAGGCACGTTTTATGAAGCGCTCTGTTCGTTTCGGCAAAAGCCGGCGGAAACGGAAGAGG
AGGCCAACGCCCAAAGGAAAGCGGCGGCTTTCTTAGAGCGGCTCGAGGGCTGGCGTACGATGGCGCGCCGCCGCTCATTA
GCTGATCTCATTTGGCTGCTGTACCGCGATACGCAATTTTACGATTTTGTCGGCGCACTGCCGGGCGGCAGGCAACGGCA
GGCGAACTTGCGCGCTTTGTACGACCGCGCGCGGCAGTACGAGTCGACGTCGTTTCGCGGCTTGTTTCGCTTTCTTCGCT
TCATCGAGCGGCTGCAAGAGCGCGGCGACGATTTAGGGGCGGCCCGGCCGCTCGGCGAGCAGGAAGACGTCGTGCGCATC
ATGACGATTCACAGCAGCAAAGGATTGGAATTTCCGATCGTGTTTCTCGCCGGGCTTGCGCGTTCGTTTCATACGCGCGA
TTTGCATCACCCGTATTTGCTTGATAAAGAGCTGGGTTTTGCCGCCCGCTTTGTCCACCCGCGGCTGCGCATCAGTTATC
CCACGCTGCCGCTGTTGGCCATCCAAACGAAAAAGCGGCTTGAACTGCTCGCTGAGGAGATGCGCATTTTGTATGTCGCG
CTCACAAGGGCAAAAGAGAAGCTGTATTTGTTGGCGTCGGTCAACGATGCGGCCAAGGAGATTGAAAAATGGAAAAGCGC
TGCTTCGGAACGCGGCTGGCTTCTCCCCGACGATGTGCGGGCTTCGGCCCGCTCCTACTTGGATTGGATCGGCCGGGCGC
TCATCCGTCATCGTGATGGGGGGGCGCTTGCGGGAATGAAGGCGCCTGAAGAGGTCGCCTCCCACCCGTCTGTATGGCGT
GTCTCCATCGTGCCGGCCGCCGATTTGCGCGGCGCAGAGGCTGCGAGGGAGGAGATGGATGGCGGCGTGCTTCTTGCCCT
CGAACAAGGACGACCCGTTCCCGTAGAAGGAGGGTGGCGGAAGGAGGTCAAACGCCGCTTGTTGTGGCGGTATTCGTATG
AGAAAGAAACGGCCGTGCGCGCCAAGCAATCGGTGTCAGAACTGAAAGAACATCGCGTGCTCTTTGGCGAGCAAGCGGAC
GAATGGCGGCCGCGACAAGGGACGGCTCCGGTGTTTTCTCGACCGCGTTTCATGCAGGAGAAAACATTGACGCCGGCCGA
AAAAGGGACGGCGCTCCATATCGTCATGCGCCATCTTGATTTGCAGGCGCCGCTTGATGAATCGTCGATCCGCAGTCAGA
TCGTCCGCCTTGTCGAAAAAGAGCTGCTGTCGGCAGAACAAGCAGAGGTGGTCGATCCAGCGGCCATCGCTGCCTTTTTC
ACTGCAGATCTTGGCCGCCGCCTTTGCGCCGCCCGCGAAGTGCACCGTGAAGTGCCGTTCAGCTTGGGGCTTAAAGCGGC
TGAACTGTACGGCGGCGAAGGGACGGAAAGCGGCCGGCGCGTCCTCGTGCAAGGGGTGATCGACTGCGTGTTTGTCGACG
AATGCGGCTATGTGATGATTGACTATAAAACGGATGAAGTCGTCCATCGTTTTGCTGGGCAAAAGGAAGAAGCGGCTCGT
TTTCTGCTCGGCCGCTACGGGACGCAAATGCGCTTGTACCGGCGGGCCATCGAGCAAATTTGGCGTGCGCCGGTGGCGGA
ATGCTATTTGTATTCGTTTGACGGCGGGTTTGTTGTGGCTGTTGAGTGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)

UvrD-like helicase C-terminal domain (UvrD_C)

(478-892)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.344

99.919

0.533

Multiple sequence alignment