Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   CWI35_RS06770 Genome accession   NZ_CP025074
Coordinates   1295602..1299105 (-) Length   1167 a.a.
NCBI ID   WP_286365221.1    Uniprot ID   -
Organism   [Bacillus] caldolyticus strain NEB414     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1290602..1304105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI35_RS06760 (CWI35_06750) - 1290674..1291852 (-) 1179 WP_021322517.1 exonuclease SbcCD subunit D -
  CWI35_RS06765 (CWI35_06755) addA 1291873..1295601 (-) 3729 WP_119877622.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  CWI35_RS06770 (CWI35_06760) addB 1295602..1299105 (-) 3504 WP_286365221.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  CWI35_RS06775 (CWI35_06765) lepB 1299329..1299877 (-) 549 WP_011230183.1 signal peptidase I -
  CWI35_RS06780 (CWI35_06770) - 1299874..1300497 (-) 624 WP_013146210.1 TVP38/TMEM64 family protein -
  CWI35_RS06785 (CWI35_06775) - 1300600..1301133 (-) 534 WP_014195018.1 competence protein ComK -
  CWI35_RS06790 (CWI35_06780) - 1301254..1301448 (-) 195 WP_011230180.1 hypothetical protein -
  CWI35_RS06795 (CWI35_06785) aceA 1301578..1302864 (-) 1287 WP_011230179.1 isocitrate lyase -
  CWI35_RS06800 (CWI35_06790) - 1303054..1303647 (+) 594 WP_014195015.1 SCO family protein -
  CWI35_RS06805 (CWI35_06795) - 1303711..1304016 (-) 306 WP_011230177.1 AzlD domain-containing protein -

Sequence

Protein


Download         Length: 1167 a.a.        Molecular weight: 134110.15 Da        Isoelectric Point: 6.8419

>NTDB_id=258020 CWI35_RS06770 WP_286365221.1 1295602..1299105(-) (addB) [[Bacillus] caldolyticus strain NEB414]
MSLRFLLGRSGSGKTAVCLEEIRRQLQEDPKGRAIVYLVPEQMTFQCEYALIHTEGVGGMIRAQVFSFTRLAWRVLQETG
GMTRYHIHDVGVQMMIRKIIEQRKQELKLFGRAADKHGFIEQLNEMITECKRYCLTPGELRRHAKAFEDGPSQPGRRLLA
DKLSDVALVYEELERSLIGHYLDSEDYLRLLAEHIPRSSYLRDADIYIDGFHHFAPQEYMIIEQLLRHCRRVTVCLTIDR
PYDDGMPDELHLFYLPAQTYRQLRELALSNDIAIEEPIVLSANRRHEDRALVHLEAQFHRRPLLPYGAKTDAVHLYEASN
RRAEIEAVAREIIRLVRDEGARYRDIALIIRQTEAYRDLVKTVFFDFGIPYFMDEKEPMHHHPLIELVRAALETVVTRWR
YEAVFRAVKTDLLFPTDGDLHMWREAADKLENYVLAYGIKGDKWTNNERWAYRRYQALDGLNVPQTDEERQFEDMLNEWR
EALAAPLRRLERRLRRAEDGRGFCMALYLFLEELQIPKKLEKMSAQAEADGRLVEARQHEQAWNAVVDLLDQYVEMLGTE
SLPLAEFVKIIEAGLDRLEFSLVPPAMDQVIVAQLDRSRLIDIKYAFVIGANDGVIPAKVKEDGLMAEVEREQLRELGVA
LAPGGREQLFYDPFFVYLALVCPSRRLYVTYPLADGEGKALMPSPLIKQLTELFPHAPVHLCGNDPFDAPAEKAEAFVTA
PRATQTYLISQLRAWKRNYGIDPLWWDVYNTFIGHRDWKEQVRHAVSALFYTNGATPLKKQWSQRLYGKKIQASVSRMEQ
FQKCPYAHFASHGLRLKERNVFRLEAPDVGQLFHAAIKQIADRLREQHLDWRELSRPDCERLSAEAVERIAPLIQQQVLS
SSHRYEYMKRKLKHVVARTTHVLSEHARASGFVPIGLELSFGPNGDLPPLRFRLPDGTVMELVGRIDRVDKAESSQGVLL
RIIDYKSSAKTLDLTEVYYGLALQMLTYLDIVLTYAEQLVGQPALPAGVLYFHIHNPIVQAKQWVDDEVEMAKKLLEPFR
MRGLLLADVEAIRLMDGRTEDGQWSLIVPAQLTKSGSIHSRSSVASPSDFAALRQHVRRLFIDIGGQIADGVVSIAPYKL
KDKTACEFCVFKPVCQFDEALSGNEYRKLAPQTKEAVIEKLAEGKEG

Nucleotide


Download         Length: 3504 bp        

>NTDB_id=258020 CWI35_RS06770 WP_286365221.1 1295602..1299105(-) (addB) [[Bacillus] caldolyticus strain NEB414]
ATGTCGCTGCGGTTTTTGCTCGGACGGTCAGGAAGTGGGAAAACGGCTGTCTGCCTCGAAGAAATTCGCCGCCAGTTGCA
AGAGGATCCAAAAGGAAGGGCGATCGTCTATCTCGTTCCGGAGCAAATGACATTTCAATGCGAATACGCGTTGATACATA
CCGAGGGAGTGGGCGGGATGATTCGCGCCCAAGTGTTCAGCTTCACCCGTCTCGCTTGGCGCGTGTTGCAGGAAACAGGC
GGCATGACCCGGTACCATATTCATGATGTTGGTGTGCAGATGATGATCCGCAAAATTATTGAGCAACGCAAACAAGAGCT
GAAACTGTTTGGCCGCGCGGCGGACAAACATGGGTTTATCGAGCAGCTGAACGAAATGATCACCGAATGCAAACGGTATT
GCCTGACGCCGGGCGAACTCCGCCGACATGCCAAGGCGTTCGAGGATGGGCCAAGCCAGCCGGGCCGCCGCTTGCTCGCT
GACAAGTTGAGCGATGTCGCGCTTGTGTATGAGGAACTTGAGCGGAGTCTTATCGGCCACTATCTTGATTCGGAAGATTA
TTTGCGCTTGCTCGCTGAGCATATTCCGCGCTCAAGTTATTTGCGGGATGCCGATATTTACATCGACGGCTTTCATCATT
TTGCGCCGCAAGAATATATGATCATCGAACAGCTTCTTCGCCATTGCCGGCGCGTCACCGTTTGTTTGACGATCGACCGC
CCGTATGACGACGGAATGCCGGACGAACTTCATCTCTTTTATTTGCCGGCGCAAACGTACCGCCAGCTGAGGGAGTTGGC
GCTAAGCAATGACATTGCGATCGAAGAGCCGATCGTGCTTTCGGCCAACCGACGCCATGAAGACAGGGCGCTCGTTCATC
TTGAGGCGCAATTTCACCGACGCCCGTTGCTTCCATATGGTGCAAAGACCGATGCCGTCCATCTTTATGAGGCGTCCAAT
CGCCGAGCGGAAATCGAAGCGGTCGCACGCGAAATCATCCGCCTTGTCCGCGATGAAGGGGCGCGCTATCGCGACATTGC
GCTGATCATCCGCCAGACGGAGGCGTACCGCGATCTGGTGAAAACGGTGTTTTTTGATTTTGGGATCCCGTATTTTATGG
ATGAAAAAGAACCGATGCACCACCACCCGCTCATTGAACTCGTGCGCGCTGCCTTAGAGACGGTCGTGACGCGTTGGCGA
TATGAAGCGGTGTTTCGGGCGGTGAAAACGGATTTGCTTTTTCCAACGGACGGCGATCTGCACATGTGGCGCGAGGCGGC
CGACAAGCTCGAAAACTATGTGCTTGCTTACGGAATCAAAGGGGATAAGTGGACAAACAATGAGCGCTGGGCGTATCGGC
GCTATCAGGCGCTTGACGGTTTGAACGTACCACAAACGGACGAAGAGCGGCAGTTTGAGGACATGCTCAATGAATGGCGC
GAGGCGTTGGCCGCTCCGCTTCGCCGGTTGGAGCGCCGTCTGCGCCGGGCGGAGGACGGACGCGGGTTTTGTATGGCATT
ATATCTGTTTTTGGAAGAATTGCAGATTCCAAAAAAACTAGAGAAAATGAGCGCACAAGCGGAAGCGGACGGCCGCCTCG
TTGAAGCGCGCCAGCATGAACAGGCGTGGAACGCCGTCGTCGATTTGCTTGACCAATATGTAGAAATGCTCGGGACAGAA
TCACTGCCGCTCGCTGAGTTTGTCAAGATTATCGAGGCTGGGCTCGATCGGTTGGAGTTCTCCCTTGTGCCGCCGGCGAT
GGACCAGGTGATCGTCGCTCAGCTTGATCGCTCTCGTTTAATTGACATCAAGTATGCGTTCGTCATCGGCGCCAACGATG
GCGTTATTCCAGCAAAAGTGAAGGAAGACGGGCTGATGGCGGAAGTGGAGCGCGAGCAGCTGCGTGAGTTGGGGGTGGCG
CTCGCCCCAGGGGGGCGGGAGCAGCTGTTTTATGACCCGTTTTTTGTCTACTTGGCACTTGTTTGTCCGAGCCGGCGGCT
GTATGTTACATATCCGCTTGCTGATGGGGAAGGGAAAGCGTTGATGCCGTCGCCGCTCATCAAGCAGCTTACGGAACTGT
TTCCGCATGCGCCGGTGCATCTGTGCGGCAACGACCCGTTCGACGCCCCGGCAGAAAAAGCAGAGGCGTTCGTGACCGCG
CCGCGGGCGACGCAAACGTATTTGATCAGCCAGCTCCGAGCGTGGAAGCGCAACTACGGCATCGATCCGCTTTGGTGGGA
TGTGTATAACACGTTCATCGGCCATCGGGACTGGAAGGAGCAGGTGCGGCATGCCGTCTCCGCGCTCTTTTATACAAACG
GAGCGACGCCGCTCAAAAAACAGTGGAGCCAGCGGCTGTACGGCAAAAAAATTCAGGCGAGCGTCTCGCGCATGGAGCAG
TTTCAAAAATGCCCGTACGCCCATTTCGCATCGCACGGGCTGCGGTTGAAAGAGCGCAACGTTTTCCGACTTGAGGCGCC
GGATGTTGGGCAATTGTTCCATGCCGCCATCAAGCAAATCGCCGACCGGCTGCGCGAACAGCATCTTGATTGGCGCGAAC
TGTCAAGACCAGACTGTGAACGGCTGTCGGCCGAAGCGGTCGAGCGAATCGCCCCGCTCATCCAGCAGCAAGTGCTCTCA
AGCTCTCATCGCTATGAATATATGAAACGAAAATTGAAACACGTCGTTGCCCGTACAACCCATGTGTTAAGTGAGCATGC
GCGGGCGAGCGGTTTCGTTCCGATCGGACTTGAGCTGTCATTCGGCCCCAATGGCGACCTGCCGCCGCTTCGGTTTCGAC
TGCCCGACGGAACAGTGATGGAGCTTGTTGGGCGGATCGACCGCGTCGATAAGGCGGAAAGCAGCCAAGGAGTGCTTCTT
CGCATTATCGATTACAAATCGAGCGCGAAAACGCTCGATTTGACGGAAGTATATTACGGCTTGGCGCTGCAAATGCTCAC
CTACCTGGACATCGTGCTGACGTATGCCGAACAGCTCGTCGGACAGCCGGCCTTGCCAGCCGGGGTGCTTTATTTTCACA
TCCACAATCCGATCGTTCAAGCGAAGCAATGGGTGGATGATGAGGTGGAAATGGCGAAAAAGTTGCTTGAACCGTTTCGG
ATGCGCGGGCTGTTGCTCGCTGACGTCGAGGCGATCCGGTTGATGGACGGCCGAACGGAAGACGGACAATGGTCGCTGAT
TGTGCCCGCTCAGCTTACGAAAAGCGGGTCGATCCATTCGCGTTCGTCGGTGGCGAGTCCATCCGACTTTGCCGCGCTCC
GCCAGCATGTCCGCCGTTTGTTCATCGACATCGGCGGGCAAATCGCCGACGGCGTCGTGTCGATCGCCCCGTACAAGTTG
AAAGATAAAACAGCGTGCGAGTTTTGCGTTTTCAAACCGGTCTGCCAATTTGATGAGGCACTCTCCGGCAACGAATACCG
GAAGCTTGCCCCGCAAACGAAGGAAGCGGTCATCGAAAAACTAGCGGAAGGGAAGGAAGGATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(309-623)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(793-1136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

50.638

100

0.51

Multiple sequence alignment