Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   CWI24_RS26140 Genome accession   NZ_CP025050
Coordinates   5391716..5393053 (+) Length   445 a.a.
NCBI ID   WP_003110427.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PB368     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5386716..5398053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI24_RS26115 (CWI24_26115) pgeF 5386859..5387587 (-) 729 WP_043085210.1 peptidoglycan editing factor PgeF -
  CWI24_RS26120 (CWI24_26120) rluD 5387584..5388546 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  CWI24_RS26125 (CWI24_26125) - 5388692..5389717 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  CWI24_RS26130 (CWI24_26130) - 5389874..5390119 (+) 246 WP_003102591.1 PP0621 family protein -
  CWI24_RS26135 (CWI24_26135) pilS 5390109..5391701 (+) 1593 WP_003110428.1 two-component system sensor histidine kinase PilS Regulator
  CWI24_RS26140 (CWI24_26140) pilR 5391716..5393053 (+) 1338 WP_003110427.1 two-component system response regulator PilR Regulator
  CWI24_RS26145 (CWI24_26145) thiO 5393099..5394193 (-) 1095 WP_033944813.1 glycine oxidase ThiO -
  CWI24_RS26150 (CWI24_26150) - 5394333..5394833 (+) 501 WP_016254062.1 GspH/FimT family pseudopilin -
  CWI24_RS26155 (CWI24_26155) - 5394956..5395444 (+) 489 WP_003141552.1 GspH/FimT family pseudopilin -
  CWI24_RS26160 (CWI24_26160) pilV 5395454..5395978 (+) 525 WP_003116261.1 type IV pilus modification protein PilV Machinery gene
  CWI24_RS26165 (CWI24_26165) - 5395987..5396802 (+) 816 WP_016562347.1 PilW family protein -
  CWI24_RS26170 (CWI24_26170) - 5396799..5397404 (+) 606 WP_003116263.1 pilus assembly PilX family protein -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49723.03 Da        Isoelectric Point: 5.8638

>NTDB_id=257749 CWI24_RS26140 WP_003110427.1 5391716..5393053(+) (pilR) [Pseudomonas aeruginosa strain PB368]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLADRI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=257749 CWI24_RS26140 WP_003110427.1 5391716..5393053(+) (pilR) [Pseudomonas aeruginosa strain PB368]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGCAGCGGCAAGGAACTGGTGGCGCGCCTGATCCACGAGCAGGGCCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAGCTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGTGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGATCGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCAGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTATACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCACTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Response regulator receiver domain (Response_reg)

(7-115)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

99.775

100

0.998
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384

Multiple sequence alignment