Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   BEN53_RS18820 Genome accession   NZ_CP029747
Coordinates   3710829..3711488 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain 2016C-3878     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3705829..3716488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN53_RS18790 (BEN53_18790) ygiS 3707112..3708719 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  BEN53_RS18800 (BEN53_18800) mqsA 3708852..3709247 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  BEN53_RS18805 (BEN53_18805) mqsR 3709249..3709545 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  BEN53_RS18810 (BEN53_18810) ygiV 3709750..3710232 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  BEN53_RS18815 (BEN53_18815) ygiW 3710285..3710677 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  BEN53_RS18820 (BEN53_18820) ciaR 3710829..3711488 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  BEN53_RS18825 (BEN53_18825) qseC 3711485..3712834 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  BEN53_RS18830 (BEN53_18830) ygiZ 3712880..3713212 (-) 333 WP_023149630.1 DUF2645 family protein -
  BEN53_RS18840 (BEN53_18840) mdaB 3713531..3714112 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  BEN53_RS18845 (BEN53_18845) ygiN 3714143..3714457 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  BEN53_RS18850 (BEN53_18850) parE 3714505..3716397 (-) 1893 WP_000195292.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=257485 BEN53_RS18820 WP_001221493.1 3710829..3711488(+) (ciaR) [Escherichia coli strain 2016C-3878]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=257485 BEN53_RS18820 WP_001221493.1 3710829..3711488(+) (ciaR) [Escherichia coli strain 2016C-3878]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365