Detailed information    

insolico Bioinformatically predicted

Overview

Name   comB4   Type   Machinery gene
Locus tag   CV729_RS01100 Genome accession   NZ_CP024947
Coordinates   218022..220385 (+) Length   787 a.a.
NCBI ID   WP_145731634.1    Uniprot ID   -
Organism   Helicobacter pylori strain J182     
Function   transformation-associated type IV transport system (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 213022..225385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CV729_RS01065 (CV729_01060) groES 213056..213412 (-) 357 WP_000671934.1 co-chaperone GroES -
  CV729_RS01075 (CV729_01070) dnaG 213687..215366 (+) 1680 WP_145731628.1 DNA primase -
  CV729_RS01080 (CV729_01075) - 215363..216415 (+) 1053 WP_145731630.1 MnmA/TRMU family protein -
  CV729_RS01085 (CV729_01080) - 216504..217331 (+) 828 WP_145731632.1 DUF5718 family protein -
  CV729_RS01090 (CV729_01085) comB2 217476..217757 (+) 282 WP_001279601.1 TrbC/VirB2 family protein Machinery gene
  CV729_RS01095 (CV729_01090) comB3 217757..218020 (+) 264 WP_000584956.1 hypothetical protein Machinery gene
  CV729_RS01100 (CV729_01095) comB4 218022..220385 (+) 2364 WP_145731634.1 VirB4 family type IV secretion/conjugal transfer ATPase Machinery gene
  CV729_RS01110 (CV729_01105) - 220687..222027 (+) 1341 WP_194151359.1 hypothetical protein -
  CV729_RS01115 (CV729_01110) cheV1 222122..223087 (+) 966 WP_120975524.1 chemotaxis protein CheV1 -
  CV729_RS01120 (CV729_01115) nspC 223084..224301 (+) 1218 WP_145731637.1 carboxynorspermidine decarboxylase -
  CV729_RS01125 (CV729_01120) lpxE 224310..224855 (-) 546 WP_227978840.1 lipid A 1-phosphatase LpxE -

Sequence

Protein


Download         Length: 787 a.a.        Molecular weight: 90238.78 Da        Isoelectric Point: 8.7109

>NTDB_id=256552 CV729_RS01100 WP_145731634.1 218022..220385(+) (comB4) [Helicobacter pylori strain J182]
MLEKLLGTIKQKVSNYFLGVLPKSYSMSEENNILGLYDEHFLLTKNENLVGILRLEGVSYTHLSTEQLQDLFTERQMALD
SLEKVVARLVVKRRKIDYKQNIQADSQYLQAILNQFENKEVYENQYFLVLESTHSLQGVLEHKKKSLMHANRENFKDILS
YKAHFLQETLKSLEIQLKNYAPKLLSSKEVLNFYAEYINGFDLPLKPLVGGYLSDSYIASSTTFEKDYFIQESFNQKTYN
RLIGIKAYESERITSIAIGALLYQETPLDIIFSIEPMSVNKTLSFLKERAKFSMSNLVKNELLEYQELVKTKRLSMQKFA
LNILIKAPSLEDLDAQTSLILGLLFKENLVGVIETFGLKGGYFSFFPERIHLNHRLRFLTSKALACLMVFERQNLGFKAN
SWGNSPLSVFKNLDYSPFLFNFHNQEVSHNNAKEIARVNGHTLIIGATGSGKSTLISFLMMSALKYQNMRLLAFDRMQGL
YSFTKFFKGHYHDGKSFSINPFCLEPNLQNLEFLQSFFLSMLDLAPSRDKEALEDMNAISSAIKSLYETLYPKDFSLLDF
KETLKRTSSNQLGLSLEPYLNNPLFNALNDAFNSNAFLNVINLDTITQNPKDLGLLAYYLFYKILEESRKNDSGFLVFLD
EFKSYVENDLLNAKINALITQARKANGVVVLALQDIYQLSGVKNAHSFLSNMGTLILYPQKNARELKHHFNVPLSETEIS
FLENTPLYARQVLVKNLGNGSSNMIDVSLESLGRYLKIFNSDSSHVNKVKALQKDYPTEWREKLLKS

Nucleotide


Download         Length: 2364 bp        

>NTDB_id=256552 CV729_RS01100 WP_145731634.1 218022..220385(+) (comB4) [Helicobacter pylori strain J182]
ATGTTAGAAAAGCTTTTAGGCACTATCAAACAAAAAGTTTCAAACTATTTTTTAGGGGTTTTGCCTAAAAGCTATTCTAT
GAGCGAAGAAAACAACATTTTAGGCTTGTATGATGAGCATTTTTTACTCACTAAAAACGAAAACTTAGTGGGCATTCTCC
GTTTAGAGGGGGTGAGTTACACCCATTTAAGCACAGAGCAATTGCAAGATCTTTTCACTGAGCGCCAAATGGCGTTGGAT
TCTTTAGAAAAGGTCGTGGCGCGCCTCGTGGTTAAAAGGCGTAAAATTGATTATAAACAAAACATTCAAGCTGACTCTCA
ATATTTACAAGCAATCTTGAATCAATTTGAAAACAAAGAAGTGTATGAGAATCAGTATTTTTTAGTTTTAGAAAGCACTC
ACTCTTTACAAGGTGTTTTGGAGCATAAGAAAAAATCCTTAATGCATGCCAATAGGGAAAATTTTAAAGACATCCTCTCT
TATAAAGCGCATTTTTTGCAAGAAACTTTAAAAAGCTTAGAAATCCAGCTCAAAAACTATGCCCCCAAACTCTTAAGCTC
CAAAGAGGTTTTGAATTTTTATGCGGAATACATTAACGGGTTTGATCTCCCTTTAAAACCCTTAGTAGGGGGGTATTTGA
GCGATAGTTATATCGCTAGTTCTACCACTTTTGAAAAAGATTATTTCATTCAAGAAAGCTTTAATCAAAAAACCTATAAC
CGCTTGATTGGCATTAAAGCTTATGAGAGCGAGCGCATCACTTCTATAGCGATCGGAGCGCTTTTATACCAAGAAACACC
TTTAGATATTATCTTTTCTATAGAGCCTATGAGCGTCAATAAAACGCTGAGTTTTTTAAAAGAGAGGGCCAAGTTTAGCA
TGTCCAATCTCGTTAAAAACGAGCTACTAGAATACCAAGAATTGGTCAAAACCAAACGCCTATCCATGCAAAAATTCGCC
TTAAACATTCTTATCAAAGCCCCTAGTTTAGAGGATTTAGACGCTCAAACGAGCTTAATTTTAGGGCTTTTATTTAAAGA
AAACTTAGTGGGCGTTATAGAAACTTTTGGCTTGAAAGGGGGGTATTTTTCCTTTTTCCCTGAACGCATCCATTTAAACC
ACCGCTTGCGTTTTTTAACCTCTAAAGCCCTAGCGTGTTTGATGGTGTTTGAAAGGCAAAATTTAGGTTTTAAGGCTAAT
TCATGGGGGAATAGCCCTTTGAGCGTGTTTAAAAATTTGGATTATTCCCCTTTTTTATTCAATTTCCACAACCAAGAAGT
GAGCCACAACAACGCCAAAGAAATCGCCAGAGTGAACGGGCATACTCTAATCATAGGGGCTACCGGGAGCGGTAAAAGCA
CGCTGATTAGCTTTTTAATGATGAGCGCTTTGAAATACCAAAACATGCGCCTTTTAGCATTTGATAGGATGCAAGGGCTG
TATTCTTTCACAAAATTTTTTAAAGGGCATTACCATGACGGCAAATCTTTTAGCATCAACCCCTTTTGTTTAGAGCCTAA
TTTGCAGAATTTAGAATTTTTGCAATCCTTCTTTTTGAGCATGTTGGATCTCGCCCCTTCAAGGGATAAAGAAGCCTTGG
AAGACATGAATGCGATTTCTAGCGCGATTAAGAGCCTTTATGAGACTTTATACCCTAAAGATTTTAGTTTGCTAGATTTT
AAAGAAACGCTTAAAAGAACCTCATCTAACCAATTGGGCTTGAGTTTAGAGCCGTATTTGAATAACCCCCTTTTTAACGC
TTTGAATGACGCATTCAACTCCAACGCTTTTTTAAATGTGATAAACCTGGACACCATCACCCAAAACCCTAAAGACTTGG
GGCTTTTAGCCTATTACTTGTTTTATAAAATCTTAGAAGAATCCAGGAAAAACGACAGCGGTTTTTTGGTTTTTTTAGAC
GAGTTTAAATCCTATGTGGAAAACGATTTACTGAACGCTAAAATTAACGCTTTAATCACGCAAGCCAGAAAAGCTAATGG
CGTGGTGGTGTTGGCTTTGCAAGATATTTACCAATTAAGCGGGGTCAAAAACGCTCATAGTTTTTTAAGCAACATGGGGA
CTCTTATTTTGTATCCGCAAAAAAACGCTAGGGAGTTGAAACACCACTTCAATGTGCCTTTGAGCGAGACTGAAATTTCT
TTTTTAGAAAACACCCCTTTGTATGCCAGGCAGGTTTTAGTCAAAAATCTGGGTAATGGGAGCTCTAACATGATTGATGT
GAGTTTGGAAAGCTTGGGGCGTTATTTGAAAATCTTTAATTCGGATTCTAGTCATGTGAATAAAGTGAAAGCGTTACAAA
AAGACTACCCTACAGAGTGGCGTGAGAAACTTTTGAAGAGCTAG

Domains

Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(176-375)

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(426-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB4 Helicobacter pylori 26695

97.713

100

0.977

Multiple sequence alignment