Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CSC33_RS21470 Genome accession   NZ_CP029660
Coordinates   4508711..4509334 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain AR_0446     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4503711..4514334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSC33_RS21450 (CSC33_4344) aroB 4504306..4505412 (-) 1107 WP_033977028.1 3-dehydroquinate synthase -
  CSC33_RS21455 (CSC33_4345) aroK 4505462..4505980 (-) 519 WP_003095833.1 shikimate kinase AroK -
  CSC33_RS21460 (CSC33_4346) pilQ 4505992..4508136 (-) 2145 WP_033977030.1 type 4a pilus secretin PilQ Machinery gene
  CSC33_RS21465 (CSC33_4347) comL/pilP 4508190..4508714 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  CSC33_RS21470 (CSC33_4348) pilO 4508711..4509334 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  CSC33_RS21475 (CSC33_4349) pilN 4509331..4509927 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  CSC33_RS21480 (CSC33_4350) pilM 4509927..4510991 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  CSC33_RS21485 (CSC33_4351) - 4511176..4513647 (+) 2472 WP_033967771.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=255944 CSC33_RS21470 WP_003103268.1 4508711..4509334(-) (pilO) [Pseudomonas aeruginosa strain AR_0446]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=255944 CSC33_RS21470 WP_003103268.1 4508711..4509334(-) (pilO) [Pseudomonas aeruginosa strain AR_0446]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGAGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401