Detailed information    

insolico Bioinformatically predicted

Overview

Name   phrF   Type   Regulator
Locus tag   CUB85_RS18640 Genome accession   NZ_CP024797
Coordinates   3715666..3715785 (+) Length   39 a.a.
NCBI ID   WP_007407639.1    Uniprot ID   A3KLD5
Organism   Bacillus velezensis strain TJ02     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context

Location: 3710666..3720785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUB85_RS18615 - 3711016..3711528 (+) 513 Protein_3579 bacteriocin biosynthesis protein AlbG -
  CUB85_RS18620 - 3711535..3712911 (-) 1377 WP_094031367.1 YncE family protein -
  CUB85_RS18625 - 3712951..3714306 (-) 1356 WP_015240776.1 YncE family protein -
  CUB85_RS18635 rapF 3714537..3715682 (+) 1146 WP_007407638.1 tetratricopeptide repeat protein Regulator
  CUB85_RS18640 phrF 3715666..3715785 (+) 120 WP_007407639.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  CUB85_RS18645 - 3715983..3716186 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  CUB85_RS18650 - 3716200..3716403 (+) 204 WP_007407641.1 hypothetical protein -
  CUB85_RS18655 - 3716438..3716623 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  CUB85_RS18660 - 3716809..3717090 (+) 282 WP_007407643.1 hypothetical protein -
  CUB85_RS18665 - 3717134..3717646 (+) 513 WP_094031368.1 stage II sporulation protein M -
  CUB85_RS18675 - 3718237..3718455 (-) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  CUB85_RS18680 speB 3718623..3719495 (-) 873 WP_007614448.1 agmatinase -
  CUB85_RS18685 speE 3719554..3720384 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 39 a.a.        Molecular weight: 4295.19 Da        Isoelectric Point: 10.0233

>NTDB_id=255532 CUB85_RS18640 WP_007407639.1 3715666..3715785(+) (phrF) [Bacillus velezensis strain TJ02]
MKLKYKLALTCLAISTVFVSAEIASAPKHQFEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=255532 CUB85_RS18640 WP_007407639.1 3715666..3715785(+) (phrF) [Bacillus velezensis strain TJ02]
ATGAAATTGAAGTATAAATTAGCACTTACTTGCTTGGCAATAAGCACTGTTTTTGTTTCAGCCGAAATCGCAAGTGCACC
GAAACATCAATTTGAAGTTGCTCAGCGCGGAATGATTTAA

Domains

Predicted by InterproScan.

Rap-phr extracellular signalling (PhrC_PhrF)

(3-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3KLD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

74.074

69.231

0.513
  phrC Bacillus subtilis subsp. subtilis str. 168

46.154

100

0.462

Multiple sequence alignment