Detailed information NTG0255531

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: CUB85_RS18635
• Genome accession: NZ_CP024797
• Coordinates: 3714537..3715682 (+)
• Length: 381 a.a.
• NCBI ID: WP_007407638.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain TJ02
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3709537..3720682

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CUB85_RS18610	-	3710434..3710862 (-)	429	WP_007407634.1	DUF1934 domain-containing protein	-
CUB85_RS18615	-	3711016..3711528 (+)	513	Protein_3579	bacteriocin biosynthesis protein AlbG	-
CUB85_RS18620	-	3711535..3712911 (-)	1377	WP_094031367.1	YncE family protein	-
CUB85_RS18625	-	3712951..3714306 (-)	1356	WP_015240776.1	YncE family protein	-
CUB85_RS18635	rapF	3714537..3715682 (+)	1146	WP_007407638.1	tetratricopeptide repeat protein	Regulator
CUB85_RS18640	phrF	3715666..3715785 (+)	120	WP_007407639.1	PhrC/PhrF family phosphatase-inhibitory pheromone	Regulator
CUB85_RS18645	-	3715983..3716186 (+)	204	WP_007407640.1	helix-turn-helix transcriptional regulator	-
CUB85_RS18650	-	3716200..3716403 (+)	204	WP_007407641.1	hypothetical protein	-
CUB85_RS18655	-	3716438..3716623 (+)	186	WP_007407642.1	DUF4177 domain-containing protein	-
CUB85_RS18660	-	3716809..3717090 (+)	282	WP_007407643.1	hypothetical protein	-
CUB85_RS18665	-	3717134..3717646 (+)	513	WP_094031368.1	stage II sporulation protein M	-
CUB85_RS18675	-	3718237..3718455 (-)	219	WP_003151056.1	helix-turn-helix transcriptional regulator	-
CUB85_RS18680	speB	3718623..3719495 (-)	873	WP_007614448.1	agmatinase	-
CUB85_RS18685	speE	3719554..3720384 (-)	831	WP_003151052.1	spermidine synthase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45543.57 Da
• Isoelectric Point: 4.5769

>NTDB_id=255531 CUB85_RS18635 WP_007407638.1 3714537..3715682(+) (rapF) [Bacillus velezensis strain TJ02]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIAEYEFLESLYVGEPDEEAI
MECFDFLRDKSMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=255531 CUB85_RS18635 WP_007407638.1 3714537..3715682(+) (rapF) [Bacillus velezensis strain TJ02]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCACAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATCGCCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATCGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_12) (189-253)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	78.478	100	0.785
rapC	Bacillus subtilis subsp. subtilis str. 168	56.579	99.738	0.564