Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   DKY66_RS01520 Genome accession   NZ_CP029561
Coordinates   241925..242353 (+) Length   142 a.a.
NCBI ID   WP_223842274.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain 32790-3A     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 236925..247353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKY66_RS01500 (DKY66_01500) - 239455..239820 (+) 366 WP_000285373.1 DUF1033 family protein -
  DKY66_RS01510 (DKY66_01510) comGA 239992..240963 (+) 972 Protein_232 competence type IV pilus ATPase ComGA -
  DKY66_RS01515 (DKY66_01515) comGB 240857..241900 (+) 1044 WP_223297804.1 competence type IV pilus assembly protein ComGB Machinery gene
  DKY66_RS01520 (DKY66_01520) comGF 241925..242353 (+) 429 WP_223842274.1 competence type IV pilus minor pilin ComGF Machinery gene
  DKY66_RS01525 (DKY66_01525) comGG 242331..242554 (+) 224 Protein_235 competence type IV pilus minor pilin ComGG -
  DKY66_RS01530 (DKY66_01530) comGH 242669..243643 (+) 975 WP_001008572.1 class I SAM-dependent methyltransferase Machinery gene
  DKY66_RS01535 (DKY66_01535) - 243675..244868 (+) 1194 WP_000047534.1 acetate kinase -
  DKY66_RS01540 (DKY66_01540) - 245019..245225 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  DKY66_RS01545 (DKY66_01545) - 245284..245421 (+) 138 WP_001869149.1 hypothetical protein -
  DKY66_RS01550 (DKY66_01550) - 245462..245917 (+) 456 WP_000905672.1 hypothetical protein -
  DKY66_RS01555 (DKY66_01555) - 245986..246651 (+) 666 WP_000008107.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 16590.29 Da        Isoelectric Point: 9.7864

>NTDB_id=254264 DKY66_RS01520 WP_223842274.1 241925..242353(+) (comGF) [Streptococcus agalactiae strain 32790-3A]
MRRLKHLHILECLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNKLYLRKQDKI
VTFGKSNKDDFRKTGYNGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide


Download         Length: 429 bp        

>NTDB_id=254264 DKY66_RS01520 WP_223842274.1 241925..242353(+) (comGF) [Streptococcus agalactiae strain 32790-3A]
ATAAGAAGGTTAAAGCATTTACACATTTTAGAATGTTTGGTAGCATTGGTTACAATCACAGGAGCTTTGCTAGTTTATCA
AGGACTAACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTTCCAGTCAGTCTGAATGGGTGTTATTAACTCAGC
AACTAAATGCAGAATTCGAAGGCGCTCACTTGGAATATTTAAGACAGAACAAACTTTATTTACGTAAGCAAGATAAGATT
GTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTATAATGGTCGAGGTTATCAACCAATGGTTTATGG
GTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTGTTTTTTATTTTAAGGACGGGTTAAAAAGGACAT
TTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(38-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Streptococcus mutans UA159

44.355

87.324

0.387
  comGF Streptococcus mutans UA140

44.355

87.324

0.387
  comGF/cglF Streptococcus pneumoniae D39

39.568

97.887

0.387
  comGF/cglF Streptococcus pneumoniae TIGR4

39.568

97.887

0.387
  comGF/cglF Streptococcus pneumoniae R6

39.568

97.887

0.387
  comGF/cglF Streptococcus pneumoniae Rx1

39.568

97.887

0.387
  comGF/cglF Streptococcus mitis NCTC 12261

40.299

94.366

0.38
  comGF/cglF Streptococcus mitis SK321

40.299

94.366

0.38

Multiple sequence alignment