Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   DKQ63_RS03730 Genome accession   NZ_CP029492
Coordinates   706159..706818 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain HS30-1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 701159..711818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKQ63_RS03700 (DKQ63_03700) parE 701249..703141 (+) 1893 WP_032235165.1 DNA topoisomerase IV subunit B -
  DKQ63_RS03705 (DKQ63_03705) ygiN 703189..703503 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  DKQ63_RS03710 (DKQ63_03710) mdaB 703534..704115 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  DKQ63_RS03720 (DKQ63_03720) ygiZ 704434..704767 (+) 334 Protein_669 DUF2645 family protein -
  DKQ63_RS03725 (DKQ63_03725) qseC 704813..706162 (-) 1350 WP_001618857.1 quorum sensing histidine kinase QseC -
  DKQ63_RS03730 (DKQ63_03730) ciaR 706159..706818 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  DKQ63_RS03735 (DKQ63_03735) ygiW 706970..707362 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  DKQ63_RS03740 (DKQ63_03740) ygiV 707415..707897 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  DKQ63_RS03745 (DKQ63_03745) mqsR 708102..708398 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  DKQ63_RS03750 (DKQ63_03750) mqsA 708400..708795 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  DKQ63_RS03760 (DKQ63_03760) ygiS 708928..710535 (+) 1608 WP_001295629.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=253910 DKQ63_RS03730 WP_001221493.1 706159..706818(-) (ciaR) [Escherichia coli strain HS30-1]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=253910 DKQ63_RS03730 WP_001221493.1 706159..706818(-) (ciaR) [Escherichia coli strain HS30-1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365