Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   CYA_RS09265 Genome accession   NC_007775
Coordinates   1976915..1978981 (+) Length   688 a.a.
NCBI ID   WP_011430784.1    Uniprot ID   Q2JT92
Organism   Synechococcus sp. JA-3-3Ab     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1971915..1983981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYA_RS09250 (CYA_1962) - 1972842..1973888 (-) 1047 WP_011430781.1 DUF1611 domain-containing protein -
  CYA_RS09255 (CYA_1963) - 1973893..1974942 (-) 1050 WP_011430782.1 dipeptide epimerase -
  CYA_RS09260 (CYA_1964) - 1975121..1976758 (+) 1638 WP_011430783.1 acetolactate synthase large subunit -
  CYA_RS09265 (CYA_1965) pilB/pilB1 1976915..1978981 (+) 2067 WP_011430784.1 GspE/PulE family protein Machinery gene
  CYA_RS09270 (CYA_1966) - 1979142..1980284 (+) 1143 WP_099834829.1 type IV pilus twitching motility protein PilT -
  CYA_RS09275 (CYA_1967) - 1980445..1981671 (+) 1227 WP_011430786.1 type II secretion system F family protein -

Sequence

Protein


Download         Length: 688 a.a.        Molecular weight: 76530.46 Da        Isoelectric Point: 6.1698

>NTDB_id=25322 CYA_RS09265 WP_011430784.1 1976915..1978981(+) (pilB/pilB1) [Synechococcus sp. JA-3-3Ab]
MSDLPSPPFTSRRAGAASKALAIANVSITPFGRKLKELGFADDKQIQDIQNALKADREGGSKALALVVKEIVGKEITPDL
ERAYKRQQLFELKIIYGIPPLDIDLEPVEISEMIGLIDSLLPLDICNRYKFLPIRRQDNQVLVAMVRPDNLQALDDIQRR
FRIQGLKLQRRVFTQRDFDALINRYMDAQAELLAIKGINDAAAPVQEEEEVVVNIAELDLDSIEDVAVDEAGEGSLEQQV
KSADDAPIIKLSNQILVKALQDGASDIHIEPQEEYLRIRFRKDGVLRQAFENFPKKIVPALTARFKIMSNLNIAERRLPQ
DGRIRRVFKGRKVDFRVSTLPSRYGEKIVLRILDNSATQLGLDKLITDPETLASFKEVVRRPFGLILVTGPTGSGKTTTL
YSALAEVNDPGINISTAEDPIEYSLPGITQVQVIREKGMDFAMILRAFLRQDPDVILVGETRDHETAKTAIEASLTGHLV
LTTLHTNDAPGAIARLTEMGIEPFMISSSLLGVLAQRLMRRVCTECRIPYHPTSEELARYGLSLSGDGEQLTFYKANKLS
PKEIEQRKATGKPICEKCGGVGYKGRVGVYEFMRMNDRLAELINKGAPTEVIKEAAVESGMKTLLAYSLMLVKQGLTTLE
EVDRVILTDKGLETELKARAKALSTCRNCGAALQVDWMDCPYCLTPKF

Nucleotide


Download         Length: 2067 bp        

>NTDB_id=25322 CYA_RS09265 WP_011430784.1 1976915..1978981(+) (pilB/pilB1) [Synechococcus sp. JA-3-3Ab]
ATGAGCGATCTTCCTTCCCCTCCCTTTACCTCGCGCCGCGCCGGCGCTGCCAGCAAAGCCCTGGCCATTGCCAACGTCAG
CATCACTCCTTTCGGCCGCAAGCTCAAAGAGCTGGGCTTTGCCGACGATAAGCAAATCCAGGACATCCAAAACGCCCTCA
AAGCCGATCGCGAAGGGGGCAGCAAGGCCCTAGCCCTGGTGGTCAAAGAGATTGTCGGCAAAGAGATCACCCCTGACCTG
GAGCGGGCCTACAAGCGGCAGCAGCTCTTTGAGCTGAAAATCATCTACGGGATCCCCCCCCTCGACATCGATTTGGAGCC
GGTGGAGATCTCGGAGATGATCGGGCTCATCGACTCGCTCTTGCCTCTCGACATCTGCAACCGCTACAAGTTCTTGCCCA
TCCGCCGCCAAGACAACCAAGTGCTGGTGGCGATGGTCAGGCCGGACAACCTGCAGGCGCTGGACGACATCCAGCGGCGC
TTCCGCATCCAGGGGCTGAAGCTGCAGCGGCGGGTCTTCACCCAGCGGGATTTCGACGCCCTGATCAACCGCTACATGGA
TGCCCAGGCGGAGCTGCTGGCCATCAAGGGCATCAACGACGCGGCTGCCCCTGTCCAAGAGGAAGAAGAGGTGGTGGTCA
ACATCGCCGAGCTCGATCTGGACAGCATCGAGGATGTCGCCGTCGATGAGGCTGGCGAGGGATCCCTGGAGCAGCAGGTC
AAATCGGCAGATGATGCCCCGATTATTAAACTGTCCAACCAGATCCTGGTCAAGGCCCTGCAGGACGGGGCGTCGGACAT
CCACATCGAGCCACAAGAAGAATACCTGCGCATCCGCTTCCGCAAGGATGGGGTGCTGCGGCAGGCTTTTGAAAACTTTC
CCAAGAAGATCGTCCCTGCCCTCACCGCTCGCTTCAAGATCATGTCCAACCTGAACATTGCCGAGCGGCGCTTGCCCCAG
GACGGGCGGATTCGCCGCGTCTTCAAGGGGCGCAAGGTGGATTTCCGCGTCAGCACCCTGCCCTCCCGCTACGGGGAGAA
GATCGTGCTGCGGATTCTGGACAACTCCGCCACCCAGCTCGGCCTGGACAAGCTGATCACCGACCCAGAGACCCTGGCCA
GCTTCAAGGAGGTTGTCAGGCGACCCTTTGGCCTGATCTTGGTAACCGGGCCCACCGGATCCGGGAAAACCACCACCCTC
TACTCGGCGCTGGCAGAAGTGAACGATCCCGGCATCAACATCAGCACCGCCGAGGATCCCATCGAATACTCCCTGCCGGG
GATCACCCAGGTGCAGGTGATCCGCGAAAAGGGCATGGACTTTGCCATGATCCTGCGCGCTTTCTTGCGGCAAGACCCGG
ATGTGATCCTGGTGGGGGAAACCCGCGACCACGAGACGGCCAAAACCGCCATCGAAGCCTCCCTCACCGGCCACCTGGTG
TTGACCACCCTGCACACCAACGATGCCCCCGGTGCCATCGCCCGTCTGACGGAGATGGGGATCGAGCCGTTCATGATCTC
CAGCTCGCTGTTGGGGGTGCTGGCCCAGCGCTTGATGCGTCGGGTATGTACCGAGTGTCGGATCCCGTACCACCCCACAT
CCGAGGAGTTGGCCCGCTATGGCCTCAGCCTCAGCGGCGACGGCGAACAGCTCACCTTCTACAAAGCCAACAAGCTCTCC
CCCAAAGAGATCGAGCAGCGCAAAGCCACCGGCAAGCCCATCTGCGAGAAATGTGGGGGCGTGGGCTACAAAGGCCGCGT
GGGGGTGTACGAGTTCATGCGCATGAACGACCGCCTGGCCGAGTTGATCAACAAGGGGGCTCCCACGGAGGTCATCAAGG
AAGCCGCTGTCGAAAGCGGCATGAAAACCCTGCTCGCCTACAGCTTGATGCTGGTAAAGCAGGGCCTGACCACGCTGGAG
GAGGTAGATCGGGTTATCCTCACCGACAAGGGTTTGGAAACGGAGCTGAAGGCCCGTGCCAAGGCCCTTAGCACCTGCCG
CAACTGTGGCGCCGCCCTGCAGGTAGACTGGATGGATTGCCCCTACTGCCTGACGCCGAAGTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(254-521)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q2JT92

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

59.644

97.965

0.584
  pilF Thermus thermophilus HB27

40.325

89.39

0.36
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.194

89.68

0.36

Multiple sequence alignment