Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   CS376_RS12875 Genome accession   NZ_CP024203
Coordinates   2610444..2611481 (-) Length   345 a.a.
NCBI ID   WP_099566903.1    Uniprot ID   -
Organism   Bacillus velezensis strain NKG-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2605444..2616481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS376_RS12825 tasA 2605678..2606463 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  CS376_RS12830 sipW 2606528..2607112 (-) 585 WP_012117977.1 signal peptidase I SipW -
  CS376_RS12835 tapA 2607084..2607755 (-) 672 WP_020954230.1 amyloid fiber anchoring/assembly protein TapA -
  CS376_RS12840 - 2608014..2608343 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  CS376_RS12845 - 2608383..2608562 (-) 180 WP_059368116.1 YqzE family protein -
  CS376_RS12850 comGG 2608619..2608996 (-) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  CS376_RS12855 comGF 2608997..2609392 (-) 396 WP_043020788.1 competence type IV pilus minor pilin ComGF -
  CS376_RS12860 comGE 2609406..2609720 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  CS376_RS12865 comGD 2609704..2610141 (-) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  CS376_RS12870 comGC 2610131..2610439 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  CS376_RS12875 comGB 2610444..2611481 (-) 1038 WP_099566903.1 competence type IV pilus assembly protein ComGB Machinery gene
  CS376_RS12880 comGA 2611468..2612538 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  CS376_RS12885 - 2612735..2613685 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  CS376_RS12890 - 2613831..2615132 (+) 1302 WP_012117986.1 hemolysin family protein -
  CS376_RS12895 - 2615170..2616000 (-) 831 WP_015240214.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39559.77 Da        Isoelectric Point: 9.4012

>NTDB_id=252912 CS376_RS12875 WP_099566903.1 2610444..2611481(-) (comGB) [Bacillus velezensis strain NKG-1]
MKRINRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLQLQLHKRQLAELTDGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGDLLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGTTAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=252912 CS376_RS12875 WP_099566903.1 2610444..2611481(-) (comGB) [Bacillus velezensis strain NKG-1]
ATGAAGCGGATTAACCGAACCTGGCCCTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGCAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCCGGCTGAGGGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCTGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGACTTGTTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATATCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTACGACTGCTTTCATCTTTGCGTTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAAAA
AAAATCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATACCACTATTAGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTATCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTGTTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAACGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614

Multiple sequence alignment