Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   CR542_RS03240 Genome accession   NZ_CP024126
Coordinates   606627..607919 (+) Length   430 a.a.
NCBI ID   WP_004194121.1    Uniprot ID   A0A140EWW0
Organism   Streptococcus suis strain HA0609     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 604462..605664 606627..607919 flank 963


Gene organization within MGE regions


Location: 604462..607919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CR542_RS03230 (CR542_03225) - 604462..605664 (+) 1203 WP_009909264.1 IS110-like element ISSsu7 family transposase -
  CR542_RS03235 (CR542_03230) - 605938..606570 (-) 633 WP_004194123.1 YigZ family protein -
  CR542_RS03240 (CR542_03235) comFA/cflA 606627..607919 (+) 1293 WP_004194121.1 DEAD/DEAH box helicase Machinery gene

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48868.45 Da        Isoelectric Point: 9.2762

>NTDB_id=252382 CR542_RS03240 WP_004194121.1 606627..607919(+) (comFA/cflA) [Streptococcus suis strain HA0609]
MKELENYYGRLFTKYQLTAKEREIAEKVPSITKKNNCFRCGTTFKEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDF
PITKCLKWKGQLTDWQQRISDGLVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYQAADNAVKEAGVQVFLTATSTDEL
DKKVRTGKLSRLSLPRRFHGNPLVVPQKVWFSKFDDTLKKNRLVPKLKKAIEEQRKSGFPLLIFVPEISKGQEFTKIMKK
TFPEETIGFVSSQTENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTASSLVQIAGRVGRSIERPTG
LLQFYHEGSTGAIEKAIAEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=252382 CR542_RS03240 WP_004194121.1 606627..607919(+) (comFA/cflA) [Streptococcus suis strain HA0609]
ATGAAAGAATTAGAAAATTATTATGGAAGATTATTTACCAAATACCAATTGACAGCAAAAGAAAGAGAAATAGCAGAAAA
AGTGCCAAGTATTACAAAAAAGAATAACTGCTTTCGCTGTGGAACAACTTTTAAAGAAGAAAACAAATTGCCAAACGATG
CTTATTACTGTCGAGCCTGCTTGCTTCTAGGCAGAGTACGGTCAGACGAAAAACTCTATCATTTTCCTCAGAAAGATTTT
CCAATCACTAAGTGTTTAAAGTGGAAAGGTCAACTAACTGATTGGCAACAAAGAATTTCAGATGGACTAGTTGCAAACGT
GGAAAATAATCGTGCGACATTGGTTCATGCAGTAACAGGAGCAGGTAAGACAGAAATGATCTACCACACCGTTGCCTCAG
TGATTGATAAAGGCGGAGCGGTTTGCCTAGCCAGTCCTCGAATTGATGTTTGTATCGAACTCTATAAACGTCTGCAAAAT
GACTTTTCAGTTCCAATTAGTTTACTACATGGAGAGTCTGAACCCTATTTCCGAACCCCATTAGTTGTAGCAACCACACA
TCAGTTATTAAAATTTTATCAGGCCTTTGATTTGGTTTTGATTGATGAAGTAGACGCCTTTCCCTATGCAGATAATCCCA
TGCTCTATCAAGCAGCAGACAATGCGGTCAAGGAAGCCGGTGTTCAAGTTTTTCTGACAGCGACTTCAACAGATGAATTG
GATAAAAAAGTCAGAACAGGTAAATTAAGTCGTCTTAGTTTGCCAAGGCGCTTTCATGGCAACCCACTTGTTGTCCCGCA
AAAAGTCTGGTTTAGTAAATTCGATGATACCCTAAAGAAAAATAGACTAGTCCCAAAGTTGAAAAAAGCGATTGAAGAAC
AGAGAAAGTCGGGCTTTCCCTTACTCATTTTTGTCCCAGAAATCTCCAAAGGTCAAGAATTTACCAAGATAATGAAAAAA
ACATTCCCAGAAGAAACAATTGGCTTTGTATCCAGTCAAACAGAAAATCGCCTTGAAATAGTTGAAGGGTTTCGCAAGAG
AGAAATCACAGTCTTAATCTCGACTACTATTCTTGAACGTGGGGTGACCTTCCCATGTGTAGACGTCTTTGTTGTTCAAG
CTAATCATTACCTCTACACAGCGTCAAGTCTTGTTCAGATTGCAGGCCGGGTCGGAAGGAGTATAGAACGTCCGACTGGT
TTACTTCAGTTTTATCATGAGGGAAGTACAGGAGCCATTGAAAAGGCAATCGCTGAAATTAAACAGATGAACAAGGAGGC
TGGTTATGTCTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(91-235)

Helicase conserved C-terminal domain (Helicase_C)

(289-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A140EWW0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

67.053

100

0.672
  comFA/cflA Streptococcus pneumoniae Rx1

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae D39

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae R6

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae TIGR4

66.357

100

0.665
  comFA/cflA Streptococcus mitis SK321

65.893

100

0.66
  comFA Lactococcus lactis subsp. cremoris KW2

54.156

92.326

0.5
  comFA Latilactobacillus sakei subsp. sakei 23K

38.051

100

0.381
  comFA Bacillus subtilis subsp. subtilis str. 168

37.59

96.512

0.363

Multiple sequence alignment