Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HCH_RS22540 Genome accession   NC_007645
Coordinates   5162967..5164181 (+) Length   404 a.a.
NCBI ID   WP_011398767.1    Uniprot ID   Q2SCC2
Organism   Hahella chejuensis KCTC 2396     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5157967..5169181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCH_RS22525 (HCH_05014) - 5158291..5159109 (+) 819 WP_049781058.1 alpha/beta fold hydrolase -
  HCH_RS32665 (HCH_05015) - 5159259..5160083 (+) 825 WP_011398765.1 OmpA family protein -
  HCH_RS22535 (HCH_05016) - 5160133..5162679 (-) 2547 WP_011398766.1 bifunctional diguanylate cyclase/phosphodiesterase -
  HCH_RS22540 (HCH_05017) pilU 5162967..5164181 (+) 1215 WP_011398767.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HCH_RS22545 (HCH_05018) - 5164416..5165792 (+) 1377 WP_011398768.1 WS/DGAT/MGAT family O-acyltransferase -
  HCH_RS22550 (HCH_05019) - 5165914..5166567 (+) 654 WP_011398769.1 DUF4291 domain-containing protein -
  HCH_RS22555 (HCH_05020) - 5166608..5167204 (-) 597 WP_011398770.1 DUF938 domain-containing protein -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 44809.11 Da        Isoelectric Point: 5.8192

>NTDB_id=25201 HCH_RS22540 WP_011398767.1 5162967..5164181(+) (pilU) [Hahella chejuensis KCTC 2396]
MEFNQYLTVLAKNDGSDLYLSTGAPPCAKFHGKLKPIDKTPLPPGRIMEIANAIMDEEQREQFAHELEMNLAYSIPQVGR
FRVNIFKQRNEVSIVARNIVTDIPNADSLGLPPILKEVLMSKRGLILFVGATGSGKSTSLAALIDHRNSNSGGHIITIED
PVEFIHKHKKSIINQREVGVDTRSYHNALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLCISTLHANNSNQALDRI
INFFPEERRNQLLMDLSLNLRAFVSQRLVPTIDGKRTAAIEILLGTPTVSELILRGDIDGIKEIMQKSENIGMQTFDAAL
FKLVQQEKISVEEALKNADSANNLRLRIKLHAGVEVPASLGDEKVEDSGPEELAAKKPSGGFSLSLQEFEEDNEEGNDPF
KPKN

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=25201 HCH_RS22540 WP_011398767.1 5162967..5164181(+) (pilU) [Hahella chejuensis KCTC 2396]
ATGGAATTCAACCAGTATCTGACCGTTCTGGCCAAGAATGACGGCTCCGATCTCTACTTGAGCACCGGCGCGCCCCCTTG
CGCCAAGTTTCATGGCAAATTGAAGCCTATCGACAAGACGCCCCTTCCACCTGGCAGAATCATGGAAATCGCCAACGCCA
TCATGGACGAAGAGCAGCGCGAGCAATTCGCCCATGAGCTGGAAATGAACCTGGCGTACAGCATCCCTCAGGTTGGCCGT
TTCCGGGTCAATATCTTCAAGCAGCGAAATGAAGTATCCATCGTCGCCCGTAATATCGTGACGGATATTCCCAACGCTGA
CAGCCTGGGGCTGCCCCCTATCCTGAAAGAAGTATTGATGTCGAAGCGGGGGCTGATTCTGTTCGTCGGCGCCACCGGCT
CCGGCAAGTCAACCTCTCTGGCGGCGCTGATTGACCACCGCAACAGCAACAGCGGCGGCCACATCATCACGATCGAAGAC
CCGGTGGAATTCATCCACAAACACAAGAAAAGCATCATCAACCAGCGCGAAGTGGGCGTCGACACGCGCAGTTACCACAA
TGCGCTGAAAAACACCCTGCGGCAGGCGCCGGATGTCATTTTGATCGGCGAGATCCGCGACCGGGAAACCATGGAGCACG
CCCTGGCCTTCGCGGAAACCGGACATCTGTGTATCTCCACCCTGCACGCCAACAACTCTAACCAGGCGCTGGACCGCATC
ATTAACTTTTTCCCAGAGGAGCGCCGCAACCAGTTGCTGATGGATTTGTCGCTTAACTTAAGGGCCTTCGTGTCGCAACG
ACTGGTGCCCACCATTGACGGCAAGCGCACCGCCGCCATTGAGATTCTGCTGGGCACGCCCACGGTCAGCGAGCTGATCC
TGCGGGGCGATATCGACGGCATCAAAGAGATCATGCAGAAGTCGGAAAACATAGGCATGCAGACCTTCGACGCCGCTCTG
TTCAAGCTGGTGCAGCAGGAAAAGATCAGCGTGGAGGAAGCCCTGAAAAACGCCGACTCCGCCAACAACCTGCGCCTGCG
CATCAAACTGCACGCAGGGGTGGAGGTTCCCGCCAGTCTGGGCGATGAGAAAGTCGAGGACTCAGGTCCGGAAGAGTTGG
CCGCCAAGAAACCCAGCGGCGGTTTTTCTCTCAGTCTGCAGGAATTTGAAGAGGACAACGAAGAAGGCAACGATCCTTTC
AAGCCTAAAAATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q2SCC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.835

86.881

0.502
  pilU Acinetobacter baylyi ADP1

56.534

87.129

0.493
  pilU Vibrio cholerae strain A1552

50

87.624

0.438

Multiple sequence alignment