Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   BAALB65_RS08445 Genome accession   NZ_CP029069
Coordinates   1635090..1637033 (+) Length   647 a.a.
NCBI ID   WP_038461444.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain ALB65     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 1630090..1642033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BAALB65_RS08420 (BAALB65_08420) def 1630464..1630946 (+) 483 WP_014417743.1 peptide deformylase -
  BAALB65_RS08425 (BAALB65_08425) fmt 1630951..1631904 (+) 954 WP_061861448.1 methionyl-tRNA formyltransferase -
  BAALB65_RS08430 (BAALB65_08430) rsmB 1631891..1633234 (+) 1344 WP_014417745.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  BAALB65_RS08435 (BAALB65_08435) rlmN 1633238..1634329 (+) 1092 WP_014417746.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  BAALB65_RS08440 (BAALB65_08440) - 1634335..1635096 (+) 762 WP_007409750.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  BAALB65_RS08445 (BAALB65_08445) stkP 1635090..1637033 (+) 1944 WP_038461444.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  BAALB65_RS08450 (BAALB65_08450) rsgA 1637048..1637938 (+) 891 WP_014417748.1 ribosome small subunit-dependent GTPase A -
  BAALB65_RS08455 (BAALB65_08455) rpe 1637940..1638590 (+) 651 WP_014417749.1 ribulose-phosphate 3-epimerase -
  BAALB65_RS08460 (BAALB65_08460) - 1638659..1639303 (+) 645 WP_025649469.1 thiamine diphosphokinase -
  BAALB65_RS08465 (BAALB65_08465) spoVM 1639389..1639469 (+) 81 WP_003154329.1 stage V sporulation protein SpoVM -
  BAALB65_RS08470 (BAALB65_08470) rpmB 1639547..1639735 (-) 189 WP_003154328.1 50S ribosomal protein L28 -
  BAALB65_RS08475 (BAALB65_08475) - 1639994..1640356 (+) 363 WP_003154327.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 647 a.a.        Molecular weight: 71683.30 Da        Isoelectric Point: 5.0695

>NTDB_id=250081 BAALB65_RS08445 WP_038461444.1 1635090..1637033(+) (stkP) [Bacillus amyloliquefaciens strain ALB65]
MLTGKRISGRYHILRPIGGGGMANVFLAEDIILEREVAIKILRFDFVNDIDFIRRFRREAQSASSLDHPNIVSIYDIGEE
GDIYYIVMEYVEGMTLKEYITAHGPLHPKEALSVMEQIVSAIAHAHQNHIVHRDIKPHNILIDHLGHIKVTDFGIATALT
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGKIPFDGESAVSIALKHLQTETPSARKWNPSIPQS
VENIILKATAKDPFHRYESAEDMEADIRTAFDAGRLNEQKFSVQDDEEMTKAIPVITDGAKAAPQAETPGETDDKEQQPA
KKKKRKWPWVLLSVCFIFIMAAVLAVTVFPSLFMPKDVSVPDVRGMKYEKAEALLEKNGLRADPDITDIEDDKIEEELMV
KTDPKAGSTVKEGSSVKLYKSIGKPKTQLIDVKGRQIGDAKKALKEKGFKHVNVKEENDDSEAGTVIDQDPSAGTDMVAS
DDEVNLTVSLGPADVTLRDLKTYSKEAASGYLEDNGLQLVEKQAHSDDVPKGQVMKQEPAAGTAVKPGSNVEVTFSLGPE
EKPAKTVKEKISIPYEPEHEGDELEVQIAIDDKDHSISDTYDSFKIKEPTEKTIELKIDQGQKGYYQVMVDHKVVSYKTI
EYPKDND

Nucleotide


Download         Length: 1944 bp        

>NTDB_id=250081 BAALB65_RS08445 WP_038461444.1 1635090..1637033(+) (stkP) [Bacillus amyloliquefaciens strain ALB65]
GTGCTAACCGGCAAGCGGATCAGCGGGCGTTATCATATCCTCCGGCCCATTGGCGGCGGCGGAATGGCAAACGTTTTTTT
AGCTGAGGACATCATTCTCGAACGTGAAGTCGCAATTAAAATTCTGCGGTTTGACTTTGTGAATGACATTGATTTTATCA
GGCGTTTCAGAAGAGAAGCACAGTCGGCATCAAGCCTCGATCATCCGAATATCGTCAGTATTTACGATATCGGGGAGGAA
GGCGATATTTATTACATTGTCATGGAATATGTGGAAGGCATGACATTAAAGGAATACATAACCGCACACGGGCCGCTTCA
TCCGAAAGAAGCGCTGTCCGTTATGGAGCAGATCGTCTCGGCCATCGCCCATGCCCATCAAAACCATATCGTGCACCGGG
ATATTAAACCGCACAATATATTGATAGATCATCTAGGGCATATCAAGGTGACGGATTTCGGCATCGCCACAGCGCTTACG
TCTACGACAATCACACATACGAATTCTGTGCTGGGCTCGGTTCATTATTTGTCTCCGGAGCAGGCGAGAGGCGGTCTGGC
AACGAAAAAATCAGATATTTACGCACTCGGCATCGTGCTGTTTGAGCTTTTAACAGGTAAGATCCCTTTTGACGGAGAAT
CAGCCGTAAGCATAGCGCTAAAGCATCTGCAAACAGAAACGCCTTCCGCCAGAAAGTGGAACCCTTCCATTCCGCAGAGC
GTCGAAAACATTATTTTAAAGGCAACCGCTAAAGACCCGTTTCACCGCTATGAAAGCGCCGAGGACATGGAAGCGGACAT
CAGAACGGCTTTTGACGCCGGCAGGCTGAATGAACAAAAATTTTCCGTTCAGGATGATGAGGAAATGACAAAAGCCATTC
CCGTTATTACTGACGGCGCAAAAGCCGCACCTCAGGCGGAAACGCCGGGGGAGACGGACGATAAGGAACAGCAGCCCGCC
AAAAAGAAAAAACGGAAATGGCCGTGGGTTTTGCTGTCCGTCTGTTTCATTTTCATTATGGCGGCTGTTCTTGCGGTCAC
CGTGTTCCCGTCTTTGTTTATGCCTAAAGATGTAAGCGTCCCCGACGTACGGGGCATGAAGTACGAAAAAGCGGAAGCAC
TGCTTGAAAAGAACGGGCTCCGGGCGGATCCTGATATAACGGACATTGAAGATGACAAAATAGAAGAGGAATTAATGGTC
AAAACGGACCCAAAAGCGGGGTCTACCGTTAAAGAAGGCTCTTCTGTAAAACTGTACAAAAGCATCGGAAAACCGAAAAC
CCAGCTGATCGACGTGAAAGGGCGGCAGATCGGCGACGCAAAAAAAGCGCTGAAGGAAAAAGGCTTCAAACATGTCAATG
TCAAAGAAGAGAACGATGACAGCGAGGCCGGCACCGTAATTGATCAGGACCCGTCAGCCGGCACCGACATGGTCGCAAGC
GACGATGAAGTCAATCTAACGGTCAGCCTCGGCCCGGCGGACGTTACGCTGAGGGACTTAAAGACTTACAGCAAGGAAGC
GGCTTCAGGGTATCTTGAAGATAACGGCCTGCAGCTTGTTGAAAAACAAGCCCATTCAGATGACGTGCCAAAAGGACAAG
TCATGAAGCAAGAGCCGGCAGCCGGCACTGCGGTTAAGCCGGGAAGTAATGTTGAAGTGACGTTCTCGCTCGGACCTGAA
GAAAAACCGGCGAAAACGGTAAAAGAAAAAATCAGCATTCCTTACGAACCCGAACATGAAGGGGACGAGCTTGAAGTGCA
AATCGCCATCGATGATAAGGACCACAGCATCTCTGATACGTATGACTCATTTAAAATAAAAGAGCCGACTGAAAAAACAA
TTGAACTGAAGATTGATCAGGGACAGAAGGGGTATTACCAAGTGATGGTCGATCATAAAGTCGTCAGCTACAAAACGATT
GAATACCCGAAAGATAATGACTGA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(358-419)

PASTA domain (PASTA)

(495-556)

PASTA domain (PASTA)

(431-489)

Protein kinase domain (Pkinase)

(11-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

39.109

93.663

0.366
  stkP Streptococcus pneumoniae D39

38.944

93.663

0.365
  stkP Streptococcus pneumoniae R6

38.944

93.663

0.365
  stkP Streptococcus pneumoniae Rx1

38.779

93.663

0.363