Detailed information NTG0249702

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CQJ38_RS12410
• Genome accession: NZ_CP023748
• Coordinates: 2532408..2533022 (-)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain LABIM40
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2527408..2538022

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CQJ38_RS12385 (CQJ38_12385)	rpsT	2527711..2527977 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
CQJ38_RS12390 (CQJ38_12390)	holA	2527994..2529035 (-)	1042	Protein_2387	DNA polymerase III subunit delta	-
CQJ38_RS19970	-	2529075..2529226 (-)	152	Protein_2388	hypothetical protein	-
CQJ38_RS12395 (CQJ38_12395)	-	2529267..2529401 (+)	135	WP_003152870.1	YqzM family protein	-
CQJ38_RS12400 (CQJ38_12400)	comEC	2529420..2531771 (-)	2352	WP_098081592.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CQJ38_RS12405 (CQJ38_12405)	-	2531772..2532341 (-)	570	WP_003152868.1	ComE operon protein 2	-
CQJ38_RS12410 (CQJ38_12410)	comEA	2532408..2533022 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
CQJ38_RS12415 (CQJ38_12415)	comER	2533081..2533902 (+)	822	WP_015240246.1	late competence protein ComER	-
CQJ38_RS12420 (CQJ38_12420)	-	2533971..2534708 (-)	738	WP_015240247.1	class I SAM-dependent DNA methyltransferase	-
CQJ38_RS12425 (CQJ38_12425)	rsfS	2534705..2535061 (-)	357	WP_007408260.1	ribosome silencing factor	-
CQJ38_RS12430 (CQJ38_12430)	yqeK	2535085..2535639 (-)	555	WP_061860724.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CQJ38_RS12435 (CQJ38_12435)	-	2535629..2536198 (-)	570	WP_098081593.1	nicotinate-nucleotide adenylyltransferase	-
CQJ38_RS12440 (CQJ38_12440)	yhbY	2536209..2536499 (-)	291	WP_098081594.1	ribosome assembly RNA-binding protein YhbY	-
CQJ38_RS12445 (CQJ38_12445)	aroE	2536493..2537335 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=249702 CQJ38_RS12410 WP_015240245.1 2532408..2533022(-) (comEA) [Bacillus velezensis strain LABIM40]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=249702 CQJ38_RS12410 WP_015240245.1 2532408..2533022(-) (comEA) [Bacillus velezensis strain LABIM40]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTAAAAGGGGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363