Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   C5B40_RS05285 Genome accession   NZ_CP028917
Coordinates   1095605..1096228 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain JB2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1090605..1101228
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5B40_RS05270 - 1091292..1093763 (-) 2472 WP_019484455.1 penicillin-binding protein 1A -
  C5B40_RS05275 pilM 1093948..1095012 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  C5B40_RS05280 pilN 1095012..1095608 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  C5B40_RS05285 pilO 1095605..1096228 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  C5B40_RS05290 pilP 1096225..1096749 (+) 525 WP_021263517.1 type 4a pilus biogenesis lipoprotein PilP -
  C5B40_RS05295 pilQ 1096803..1098947 (+) 2145 WP_003114575.1 type 4a pilus secretin PilQ Machinery gene
  C5B40_RS05300 aroK 1098959..1099477 (+) 519 WP_003095833.1 shikimate kinase AroK -
  C5B40_RS05305 aroB 1099527..1100633 (+) 1107 WP_003112148.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=249306 C5B40_RS05285 WP_003103268.1 1095605..1096228(+) (pilO) [Pseudomonas aeruginosa strain JB2]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=249306 C5B40_RS05285 WP_003103268.1 1095605..1096228(+) (pilO) [Pseudomonas aeruginosa strain JB2]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTTGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTATCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401