Detailed information NTG0024838

Bioinformatically predicted

Overview

• Name: comA
• Type: Machinery gene
• Locus tag: PFL01_RS20970
• Genome accession: NC_007492
• Coordinates: 4714320..4716563 (-)
• Length: 747 a.a.
• NCBI ID: WP_011335451.1
• Uniprot ID: Q3K8I9
• Organism: Pseudomonas fluorescens Pf0-1
• Function: ssDNA transport through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4709320..4721563

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PFL01_RS20935 (Pfl01_4171)	murB	4709720..4710739 (-)	1020	WP_011335447.1	UDP-N-acetylmuramate dehydrogenase	-
PFL01_RS20940 (Pfl01_4172)	-	4710736..4711200 (-)	465	WP_007950772.1	low molecular weight protein-tyrosine-phosphatase	-
PFL01_RS20945 (Pfl01_4173)	kdsB	4711200..4711964 (-)	765	WP_011335448.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
PFL01_RS20950 (Pfl01_4174)	-	4711961..4712146 (-)	186	WP_003179363.1	Trm112 family protein	-
PFL01_RS20955 (Pfl01_4175)	lpxK	4712171..4713181 (-)	1011	WP_011335449.1	tetraacyldisaccharide 4'-kinase	-
PFL01_RS20960 (Pfl01_4176)	-	4713181..4713609 (-)	429	WP_007950767.1	ExbD/TolR family protein	-
PFL01_RS20965 (Pfl01_4177)	exbB	4713606..4714241 (-)	636	WP_011335450.1	MotA/TolQ/ExbB proton channel family protein	Machinery gene
PFL01_RS20970 (Pfl01_4178)	comA	4714320..4716563 (-)	2244	WP_011335451.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PFL01_RS20975 (Pfl01_4179)	-	4716700..4717218 (+)	519	WP_011335452.1	DUF2062 domain-containing protein	-
PFL01_RS20980 (Pfl01_4180)	-	4717273..4718052 (-)	780	WP_011335453.1	ABC transporter permease	-
PFL01_RS20985 (Pfl01_4181)	-	4718049..4718981 (-)	933	WP_011335454.1	ABC transporter ATP-binding protein	-
PFL01_RS20990 (Pfl01_4182)	-	4719115..4719738 (-)	624	WP_041475399.1	glutathione S-transferase family protein	-
PFL01_RS20995 (Pfl01_4183)	-	4719782..4720441 (-)	660	WP_041475400.1	transglutaminase-like domain-containing protein	-

Sequence

Protein

• Length: 747 a.a.
• Molecular weight: 81771.89 Da
• Isoelectric Point: 9.9792

>NTDB_id=24838 PFL01_RS20970 WP_011335451.1 4714320..4716563(-) (comA) [Pseudomonas fluorescens Pf0-1]
MRTGMMALAVGLLAPVFLPALPPVGLLLLLPWVALMLLPFRSYPLAFLLLGFTWASFNAQLALNDRLPSRLDGETRWVEG
RVVGLPQNAEGVVRFELADARSRHEKLPSLMRLAWYAGPEIKSGERWRLAVKLKRPGGLLNPDAFDYEAWLLAQRIGATG
TVKDGQRLAPAQWAWRDSIRQRLLAVDAQGRNGALAALVLGDGSGLGREDWQILQDTGTVHLLVISGQHIGLLAALMYAL
VVGLARVGIWPLRWPWLPWACGLAFAAALGYGLLAGFDVPVRRACVMVALVLLWRLRFRHLGAWWPLLLAFDGVLLMDPL
ASLRPGLWLSFVAVAVLIFTFGGRLGPWRWWQTWTRAQWLIAIGLCPVLLALSLPISLSGPLANLLAVPCVSFAVLPPAL
LGTLLLPIPYVGEGLLWLAGGLIDGLFRALALIAGRWPAWIAASIPGWVLALGCIGALLLLLPRGVPLRLLGWPLLLVLA
FPPRERLSEGVADVWQLDVGQGLAILIRTRHHTLLYDSGPRFGDFDLGERVVLPALRKLGVEHLDLMLLSHADADHAGGA
LAVAKGLPVSRVISGDPSGLAEALNAEACESGRQWQWDGVAFHLWQWTDAHDSNQRSCVLQIEANGERLLLSGDIDSAAE
RDLLNSALAVHTQWLQAPHHGSRSSSSMALLKALQPQAVLISRGQGNSFGHPHPTVIARYRKQGIQIHDSAEQGAIHLQL
GRFQPARSMRQQRRFWRDPPLPGAEYR

Nucleotide

• Length: 2244 bp

>NTDB_id=24838 PFL01_RS20970 WP_011335451.1 4714320..4716563(-) (comA) [Pseudomonas fluorescens Pf0-1]
ATGCGCACAGGGATGATGGCGCTGGCGGTCGGTCTGCTGGCTCCGGTTTTTTTACCGGCCTTGCCGCCGGTCGGGTTATT
GCTGCTGTTGCCGTGGGTGGCGCTGATGCTGCTGCCGTTTCGCAGCTATCCACTGGCTTTTTTACTGCTTGGATTCACAT
GGGCGAGTTTCAACGCGCAGTTGGCGTTGAATGATCGACTGCCTTCCCGGCTGGATGGAGAAACACGCTGGGTGGAAGGA
CGGGTCGTCGGCTTGCCGCAGAATGCCGAGGGCGTCGTGCGCTTCGAACTGGCCGATGCGCGCTCGCGTCACGAGAAACT
GCCGTCGCTGATGCGTCTGGCGTGGTACGCCGGGCCTGAGATCAAAAGTGGCGAACGCTGGCGACTGGCGGTCAAGCTCA
AGCGTCCCGGCGGCCTGCTCAATCCGGATGCGTTCGATTACGAAGCCTGGCTGCTGGCGCAGCGCATCGGTGCCACCGGG
ACGGTGAAAGATGGCCAGCGTCTGGCGCCGGCGCAGTGGGCCTGGCGCGACAGTATTCGCCAGCGCCTGCTTGCGGTGGA
TGCCCAGGGACGTAACGGCGCCCTGGCTGCGTTGGTCCTCGGTGATGGTTCGGGTCTCGGCCGCGAAGACTGGCAGATAT
TGCAGGACACCGGCACCGTGCATCTGCTGGTGATTTCCGGTCAGCACATCGGGCTGTTGGCTGCGTTGATGTATGCGCTG
GTCGTCGGGCTGGCGCGGGTCGGGATCTGGCCGTTGCGTTGGCCCTGGCTGCCCTGGGCGTGTGGTCTGGCGTTCGCGGC
GGCGCTGGGTTACGGACTGCTGGCCGGGTTCGACGTGCCGGTACGGCGAGCCTGCGTGATGGTTGCGCTGGTGCTGTTGT
GGCGTTTGCGCTTCCGCCACCTCGGTGCATGGTGGCCATTGCTGTTGGCGTTCGACGGCGTGTTGCTGATGGATCCGCTG
GCCAGCCTGCGCCCGGGGTTGTGGCTGTCGTTCGTGGCGGTGGCTGTGTTGATCTTCACCTTCGGCGGCCGTCTGGGGCC
GTGGCGCTGGTGGCAGACCTGGACGCGCGCGCAGTGGCTGATCGCGATTGGTTTGTGCCCGGTGCTGCTGGCGCTGAGCC
TGCCGATCAGTCTCAGCGGGCCGCTGGCCAATCTGCTGGCGGTGCCATGCGTCAGTTTTGCGGTGCTGCCGCCAGCGTTG
CTGGGCACATTGTTGCTGCCGATCCCTTATGTCGGGGAAGGGCTGCTGTGGCTGGCGGGCGGACTGATCGATGGATTGTT
CCGGGCGCTGGCCCTGATTGCCGGGCGGTGGCCGGCGTGGATTGCGGCGTCGATACCGGGATGGGTTCTGGCGCTGGGTT
GCATCGGTGCACTGCTGTTATTGCTGCCCCGAGGCGTACCCCTGCGCCTCTTAGGCTGGCCATTGCTGCTGGTGCTGGCA
TTTCCGCCTCGGGAACGCCTGTCCGAAGGCGTGGCCGATGTCTGGCAACTGGACGTCGGTCAGGGCCTGGCGATTCTGAT
CCGCACCCGGCATCACACCTTGCTGTACGACAGTGGCCCGCGTTTCGGCGACTTCGACCTCGGCGAGCGAGTGGTGCTGC
CGGCATTGCGCAAACTGGGCGTGGAACACCTCGACCTGATGCTGCTCAGCCATGCCGATGCCGATCATGCAGGTGGCGCA
CTCGCGGTGGCGAAAGGATTGCCGGTCAGTCGGGTCATCAGCGGCGATCCGTCGGGGCTAGCCGAAGCGCTGAACGCCGA
GGCTTGTGAAAGTGGTCGGCAATGGCAGTGGGACGGCGTGGCCTTTCATCTATGGCAGTGGACCGACGCCCATGACAGCA
ACCAGCGTTCCTGCGTGTTGCAGATCGAAGCCAATGGCGAACGGTTGCTGCTGAGCGGTGACATCGACAGCGCCGCCGAA
CGGGACCTGCTCAACAGCGCACTGGCAGTTCATACCCAATGGCTACAAGCCCCCCACCATGGCAGCCGCAGTTCTTCGTC
GATGGCCTTGCTCAAGGCTTTGCAGCCACAGGCCGTGCTGATTTCCCGAGGGCAGGGCAATTCGTTCGGCCACCCGCACC
CGACGGTCATCGCCCGCTACCGCAAACAGGGCATACAAATCCATGACAGCGCCGAGCAGGGTGCCATTCATCTGCAACTG
GGGCGGTTTCAGCCGGCCCGGTCGATGCGTCAACAACGCCGGTTCTGGCGCGACCCGCCGTTGCCGGGGGCGGAGTACCG
TTGA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (498-681)

• Competence protein (Competence) (198-464)

• Domain of unknown function (DUF4131) (DUF4131) (24-167)

3D structure

• AlphaFold DB: Q3K8I9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comA	Pseudomonas stutzeri DSM 10701	63.004	96.252	0.606
comA	Ralstonia pseudosolanacearum GMI1000	35.369	100	0.372