Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   AOY58_RS19375 Genome accession   NZ_CP028767
Coordinates   4082659..4083318 (+) Length   219 a.a.
NCBI ID   WP_001221488.1    Uniprot ID   -
Organism   Escherichia coli strain A7     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4077659..4088318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOY58_RS19355 (AOY58_20640) plsC 4077806..4078543 (-) 738 WP_000965717.1 1-acylglycerol-3-phosphate O-acyltransferase -
  AOY58_RS19360 (AOY58_20645) parC 4078776..4081034 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  AOY58_RS19365 (AOY58_20655) ygiV 4081580..4082062 (-) 483 WP_097343956.1 GyrI-like domain-containing protein -
  AOY58_RS19370 (AOY58_20660) ygiW 4082115..4082507 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  AOY58_RS19375 (AOY58_20665) ciaR 4082659..4083318 (+) 660 WP_001221488.1 quorum sensing response regulator transcription factor QseB Regulator
  AOY58_RS19380 (AOY58_20670) qseC 4083315..4084664 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  AOY58_RS19385 (AOY58_20675) ygiZ 4084710..4085042 (-) 333 WP_000912123.1 DUF2645 family protein -
  AOY58_RS19390 (AOY58_20680) mdaB 4085361..4085942 (+) 582 WP_000065440.1 NADPH:quinone oxidoreductase MdaB -
  AOY58_RS19395 (AOY58_20685) ygiN 4085973..4086287 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  AOY58_RS19400 (AOY58_20690) - 4086331..4087662 (-) 1332 WP_000778880.1 MFS transporter -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24692.61 Da        Isoelectric Point: 6.3527

>NTDB_id=248018 AOY58_RS19375 WP_001221488.1 4082659..4083318(+) (ciaR) [Escherichia coli strain A7]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQCEPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=248018 AOY58_RS19375 WP_001221488.1 4082659..4083318(+) (ciaR) [Escherichia coli strain A7]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGTGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365