Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   AOY74_RS19005 Genome accession   NZ_CP028754
Coordinates   3966996..3967655 (+) Length   219 a.a.
NCBI ID   WP_022296675.1    Uniprot ID   -
Organism   Escherichia coli strain A21     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3961996..3972655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOY74_RS18990 (AOY74_20005) ygiS 3964201..3965808 (-) 1608 WP_033556292.1 ABC transporter substrate-binding protein -
  AOY74_RS18995 (AOY74_20010) ygiV 3965917..3966399 (-) 483 WP_000183484.1 GyrI-like domain-containing protein -
  AOY74_RS19000 (AOY74_20015) ygiW 3966452..3966844 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  AOY74_RS19005 (AOY74_20020) ciaR 3966996..3967655 (+) 660 WP_022296675.1 quorum sensing response regulator transcription factor QseB Regulator
  AOY74_RS19010 (AOY74_20025) qseC 3967652..3969001 (+) 1350 WP_000673367.1 quorum sensing histidine kinase QseC -
  AOY74_RS19015 (AOY74_20030) mdaB 3969111..3969692 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  AOY74_RS19020 (AOY74_20035) ygiN 3969723..3970037 (+) 315 WP_000633738.1 putative quinol monooxygenase -
  AOY74_RS19025 (AOY74_20040) - 3970082..3970969 (-) 888 WP_000614945.1 MurR/RpiR family transcriptional regulator -
  AOY74_RS19030 (AOY74_20045) - 3970966..3971913 (-) 948 WP_001296418.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24701.65 Da        Isoelectric Point: 6.9848

>NTDB_id=247055 AOY74_RS19005 WP_022296675.1 3966996..3967655(+) (ciaR) [Escherichia coli strain A21]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVVARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGDPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=247055 AOY74_RS19005 WP_022296675.1 3966996..3967655(+) (ciaR) [Escherichia coli strain A21]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTATTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGTCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGACCCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365