Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   AOY76_RS03665 Genome accession   NZ_CP028752
Coordinates   749694..750353 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain A23     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 744694..755353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOY76_RS03640 (AOY76_03925) parE 744785..746677 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  AOY76_RS03645 (AOY76_03930) ygiN 746725..747039 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  AOY76_RS03650 (AOY76_03935) mdaB 747070..747651 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  AOY76_RS03655 (AOY76_03945) ygiZ 747970..748302 (+) 333 WP_000917684.1 DUF2645 family protein -
  AOY76_RS03660 (AOY76_03950) qseC 748348..749697 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  AOY76_RS03665 (AOY76_03955) ciaR 749694..750353 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  AOY76_RS03670 (AOY76_03960) ygiW 750486..750896 (+) 411 WP_112032700.1 OB fold stress tolerance protein YgiW -
  AOY76_RS03675 (AOY76_03965) ygiV 750949..751431 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  AOY76_RS03680 (AOY76_03970) mqsR 751636..751932 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  AOY76_RS03685 (AOY76_03975) mqsA 751934..752329 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  AOY76_RS03690 (AOY76_03985) ygiS 752462..754069 (+) 1608 WP_001295629.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=246847 AOY76_RS03665 WP_001221493.1 749694..750353(-) (ciaR) [Escherichia coli strain A23]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=246847 AOY76_RS03665 WP_001221493.1 749694..750353(-) (ciaR) [Escherichia coli strain A23]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365