Detailed information NTG0246511

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: CNH35_RS08290
• Genome accession: NZ_CP023390
• Coordinates: 1613623..1614120 (-)
• Length: 165 a.a.
• NCBI ID: WP_001789864.1
• Uniprot ID: -
• Organism: Staphylococcus aureus subsp. aureus str. Newman strain NYU_Newman
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1608623..1619120

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CNH35_RS08265 (CNH35_08230)	gcvPB	1608785..1610257 (-)	1473	WP_000202192.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
CNH35_RS08270 (CNH35_08235)	gcvPA	1610250..1611596 (-)	1347	WP_000019687.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
CNH35_RS08275 (CNH35_08240)	gcvT	1611616..1612707 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
CNH35_RS08280 (CNH35_08245)	-	1612866..1613390 (-)	525	WP_001015117.1	shikimate kinase	-
CNH35_RS08285 (CNH35_08250)	-	1613380..1613526 (-)	147	WP_001789879.1	hypothetical protein	-
CNH35_RS08290 (CNH35_08255)	comGF	1613623..1614120 (-)	498	WP_001789864.1	competence type IV pilus minor pilin ComGF	Machinery gene
CNH35_RS08295 (CNH35_08260)	comGE	1614038..1614337 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
CNH35_RS08300 (CNH35_08265)	comGD	1614324..1614770 (-)	447	WP_001790850.1	competence type IV pilus minor pilin ComGD	Machinery gene
CNH35_RS08305 (CNH35_08270)	comGC	1614748..1615059 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
CNH35_RS08310 (CNH35_08275)	comGB	1615073..1616143 (-)	1071	WP_000775708.1	competence type IV pilus assembly protein ComGB	Machinery gene
CNH35_RS08315 (CNH35_08280)	comGA	1616115..1617089 (-)	975	WP_000697228.1	competence type IV pilus ATPase ComGA	Machinery gene
CNH35_RS08320 (CNH35_08285)	-	1617141..1617764 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
CNH35_RS08325 (CNH35_08290)	-	1617761..1618090 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
CNH35_RS08330 (CNH35_08295)	-	1618090..1619076 (-)	987	WP_000161314.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19164.88 Da
• Isoelectric Point: 10.2192

>NTDB_id=246511 CNH35_RS08290 WP_001789864.1 1613623..1614120(-) (comGF) [Staphylococcus aureus subsp. aureus str. Newman strain NYU_Newman]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=246511 CNH35_RS08290 WP_001789864.1 1613623..1614120(-) (comGF) [Staphylococcus aureus subsp. aureus str. Newman strain NYU_Newman]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (67-155)

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus MW2	100	100	1
comGF	Staphylococcus aureus N315	96.364	100	0.964