Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CLI97_RS03380 Genome accession   NZ_CP023320
Coordinates   617465..618079 (-) Length   204 a.a.
NCBI ID   WP_013352942.1    Uniprot ID   A0A411A874
Organism   Bacillus velezensis strain SCGB     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 612465..623079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLI97_RS03355 (CLI97_00677) rpsT 612765..613031 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  CLI97_RS03360 (CLI97_00678) holA 613048..614089 (-) 1042 Protein_676 DNA polymerase III subunit delta -
  CLI97_RS20750 - 614129..614283 (-) 155 Protein_677 hypothetical protein -
  CLI97_RS03365 - 614324..614458 (+) 135 WP_003152870.1 YqzM family protein -
  CLI97_RS03370 (CLI97_00679) comEC 614477..616828 (-) 2352 WP_094247717.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  CLI97_RS03375 (CLI97_00680) - 616829..617398 (-) 570 WP_003152868.1 ComE operon protein 2 -
  CLI97_RS03380 (CLI97_00681) comEA 617465..618079 (-) 615 WP_013352942.1 helix-hairpin-helix domain-containing protein Machinery gene
  CLI97_RS03385 (CLI97_00682) comER 618138..618959 (+) 822 WP_014305449.1 late competence protein ComER -
  CLI97_RS03390 (CLI97_00683) - 619028..619765 (-) 738 WP_031378935.1 class I SAM-dependent methyltransferase -
  CLI97_RS03395 (CLI97_00684) rsfS 619762..620118 (-) 357 WP_003152864.1 ribosome silencing factor -
  CLI97_RS03400 (CLI97_00685) yqeK 620136..620696 (-) 561 WP_032856887.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  CLI97_RS03405 (CLI97_00686) - 620686..621255 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  CLI97_RS03410 (CLI97_00687) yhbY 621266..621556 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  CLI97_RS03415 (CLI97_00688) aroE 621550..622392 (-) 843 WP_094247716.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22190.27 Da        Isoelectric Point: 5.4841

>NTDB_id=246019 CLI97_RS03380 WP_013352942.1 617465..618079(-) (comEA) [Bacillus velezensis strain SCGB]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=246019 CLI97_RS03380 WP_013352942.1 617465..618079(-) (comEA) [Bacillus velezensis strain SCGB]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment