Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   AOY92_RS03665 Genome accession   NZ_CP028737
Coordinates   752983..753642 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain A39     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 747983..758642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOY92_RS03640 (AOY92_03915) yqiA 748051..748632 (+) 582 WP_000105733.1 esterase YqiA -
  AOY92_RS03645 (AOY92_03920) parE 748661..750553 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  AOY92_RS03650 (AOY92_03925) ygiN 750601..750915 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  AOY92_RS03655 (AOY92_03930) mdaB 750946..751527 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  AOY92_RS03660 (AOY92_03935) qseC 751637..752986 (-) 1350 WP_023140511.1 quorum sensing histidine kinase QseC -
  AOY92_RS03665 (AOY92_03940) ciaR 752983..753642 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  AOY92_RS03670 (AOY92_03945) ygiW 753794..754186 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  AOY92_RS03675 (AOY92_03950) ygiV 754239..754721 (+) 483 WP_240757973.1 GyrI-like domain-containing protein -
  AOY92_RS03680 (AOY92_03960) parC 755267..757525 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  AOY92_RS03685 (AOY92_03965) plsC 757759..758497 (+) 739 Protein_722 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=245632 AOY92_RS03665 WP_001221502.1 752983..753642(-) (ciaR) [Escherichia coli strain A39]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=245632 AOY92_RS03665 WP_001221502.1 752983..753642(-) (ciaR) [Escherichia coli strain A39]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365