Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CLD04_RS13455 Genome accession   NZ_CP023257
Coordinates   2511976..2512593 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain TLO3     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2506976..2517593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLD04_RS13430 (CLD04_13430) rpsT 2507278..2507544 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  CLD04_RS13435 (CLD04_13435) holA 2507559..2508602 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  CLD04_RS21660 - 2508642..2508791 (-) 150 WP_120363468.1 hypothetical protein -
  CLD04_RS13440 (CLD04_13440) yqzM 2508832..2508966 (+) 135 WP_003229983.1 YqzM family protein -
  CLD04_RS13445 (CLD04_13445) comEC 2509006..2511336 (-) 2331 WP_095843844.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  CLD04_RS13450 (CLD04_13450) comEB 2511340..2511909 (-) 570 WP_003229978.1 ComE operon protein 2 -
  CLD04_RS13455 (CLD04_13455) comEA 2511976..2512593 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  CLD04_RS13460 (CLD04_13460) comER 2512677..2513498 (+) 822 WP_014480313.1 late competence protein ComER -
  CLD04_RS13465 (CLD04_13465) yqeM 2513564..2514307 (-) 744 WP_029317885.1 class I SAM-dependent methyltransferase -
  CLD04_RS13470 (CLD04_13470) rsfS 2514304..2514660 (-) 357 WP_014480315.1 ribosome silencing factor -
  CLD04_RS13475 (CLD04_13475) yqeK 2514678..2515238 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  CLD04_RS13480 (CLD04_13480) nadD 2515228..2515797 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  CLD04_RS13485 (CLD04_13485) yhbY 2515809..2516099 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  CLD04_RS13490 (CLD04_13490) aroE 2516093..2516935 (-) 843 WP_041351695.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=245560 CLD04_RS13455 WP_004398514.1 2511976..2512593(-) (comEA) [Bacillus subtilis strain TLO3]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=245560 CLD04_RS13455 WP_004398514.1 2511976..2512593(-) (comEA) [Bacillus subtilis strain TLO3]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment