Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   AOY60_RS19945 Genome accession   NZ_CP028732
Coordinates   4105539..4106198 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain H3     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4100539..4111198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOY60_RS19920 (AOY60_21210) ygiS 4101822..4103429 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  AOY60_RS19925 (AOY60_21220) mqsA 4103562..4103957 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  AOY60_RS19930 (AOY60_21225) mqsR 4103959..4104255 (-) 297 Protein_3907 type II toxin-antitoxin system toxin MqsR -
  AOY60_RS19935 (AOY60_21230) ygiV 4104460..4104942 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  AOY60_RS19940 (AOY60_21235) ygiW 4104995..4105387 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  AOY60_RS19945 (AOY60_21240) ciaR 4105539..4106198 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  AOY60_RS19950 (AOY60_21245) qseC 4106195..4107544 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  AOY60_RS19955 (AOY60_21250) ygiZ 4107590..4107922 (-) 333 WP_000917684.1 DUF2645 family protein -
  AOY60_RS19960 (AOY60_21260) mdaB 4108241..4108822 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  AOY60_RS19965 (AOY60_21265) ygiN 4108853..4109167 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  AOY60_RS19970 (AOY60_21270) parE 4109215..4111107 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=245358 AOY60_RS19945 WP_001221493.1 4105539..4106198(+) (ciaR) [Escherichia coli strain H3]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=245358 AOY60_RS19945 WP_001221493.1 4105539..4106198(+) (ciaR) [Escherichia coli strain H3]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment