Detailed information NTG0244784

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CK238_RS12600
• Genome accession: NZ_CP023075
• Coordinates: 2533648..2534262 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain K26
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2528648..2539262

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CK238_RS12575 (CK238_12590)	rpsT	2528951..2529217 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
CK238_RS12580 (CK238_12595)	holA	2529180..2530275 (-)	1096	Protein_2408	DNA polymerase III subunit delta	-
CK238_RS20760	-	2530315..2530466 (-)	152	Protein_2409	hypothetical protein	-
CK238_RS12585 (CK238_12600)	-	2530507..2530641 (+)	135	WP_003152870.1	YqzM family protein	-
CK238_RS12590 (CK238_12605)	comEC	2530660..2533011 (-)	2352	WP_017418179.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CK238_RS12595 (CK238_12610)	-	2533012..2533581 (-)	570	WP_003152868.1	ComE operon protein 2	-
CK238_RS12600 (CK238_12615)	comEA	2533648..2534262 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
CK238_RS12605 (CK238_12620)	comER	2534321..2535142 (+)	822	WP_014305449.1	late competence protein ComER	-
CK238_RS12610 (CK238_12625)	-	2535211..2535948 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
CK238_RS12615 (CK238_12630)	rsfS	2535945..2536301 (-)	357	WP_003152864.1	ribosome silencing factor	-
CK238_RS12620 (CK238_12635)	yqeK	2536298..2536879 (-)	582	WP_017418181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CK238_RS12625 (CK238_12640)	-	2536869..2537438 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
CK238_RS12630 (CK238_12645)	yhbY	2537449..2537739 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
CK238_RS12635 (CK238_12650)	aroE	2537733..2538575 (-)	843	WP_017418183.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=244784 CK238_RS12600 WP_013352942.1 2533648..2534262(-) (comEA) [Bacillus velezensis strain K26]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=244784 CK238_RS12600 WP_013352942.1 2533648..2534262(-) (comEA) [Bacillus velezensis strain K26]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363