Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CKG20_RS09455 Genome accession   NZ_CP023047
Coordinates   1809263..1809742 (-) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain USDA-ARS-USMARC-186     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1804263..1814742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKG20_RS09420 (CKG20_09420) lptE 1804543..1805031 (+) 489 WP_006248408.1 LPS assembly lipoprotein LptE -
  CKG20_RS09425 (CKG20_09425) holA 1805074..1806099 (+) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  CKG20_RS09430 (CKG20_09430) yacG 1806100..1806285 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  CKG20_RS09435 (CKG20_09435) coaE 1806267..1806902 (-) 636 WP_006248405.1 dephospho-CoA kinase -
  CKG20_RS09440 (CKG20_09440) - 1806938..1807594 (-) 657 WP_006248404.1 prepilin peptidase -
  CKG20_RS09445 (CKG20_09445) pilC 1807597..1808793 (-) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  CKG20_RS09450 (CKG20_09450) - 1808799..1809242 (-) 444 Protein_1841 pilus assembly protein PilB -
  CKG20_RS09455 (CKG20_09455) pilA 1809263..1809742 (-) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  CKG20_RS09460 (CKG20_09460) radA 1809923..1811302 (+) 1380 WP_006248399.1 DNA repair protein RadA -
  CKG20_RS09465 (CKG20_09465) - 1811357..1811587 (-) 231 WP_006248398.1 YgjV family protein -
  CKG20_RS09470 (CKG20_09470) folA 1811707..1812201 (-) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  CKG20_RS09475 (CKG20_09475) proB 1812313..1813397 (+) 1085 Protein_1846 glutamate 5-kinase -
  CKG20_RS09480 (CKG20_09480) - 1813397..1814161 (+) 765 WP_006248395.1 TSUP family transporter -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=244481 CKG20_RS09455 WP_006248401.1 1809263..1809742(-) (pilA) [Mannheimia haemolytica strain USDA-ARS-USMARC-186]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=244481 CKG20_RS09455 WP_006248401.1 1809263..1809742(-) (pilA) [Mannheimia haemolytica strain USDA-ARS-USMARC-186]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491

Multiple sequence alignment