Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CKG21_RS04320 Genome accession   NZ_CP023046
Coordinates   844261..844740 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain USDA-ARS-USMARC-187     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 839261..849740
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKG21_RS04295 (CKG21_04295) - 839842..840606 (-) 765 WP_006248395.1 TSUP family transporter -
  CKG21_RS04300 (CKG21_04300) proB 840606..841690 (-) 1085 Protein_816 glutamate 5-kinase -
  CKG21_RS04305 (CKG21_04305) folA 841802..842296 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  CKG21_RS04310 (CKG21_04310) - 842416..842646 (+) 231 WP_006248398.1 YgjV family protein -
  CKG21_RS04315 (CKG21_04315) radA 842701..844080 (-) 1380 WP_006248399.1 DNA repair protein RadA -
  CKG21_RS04320 (CKG21_04320) pilA 844261..844740 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  CKG21_RS04325 (CKG21_04325) - 844761..845204 (+) 444 Protein_821 pilus assembly protein PilB -
  CKG21_RS04330 (CKG21_04330) pilC 845210..846406 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  CKG21_RS04335 (CKG21_04335) - 846409..847065 (+) 657 WP_006248404.1 prepilin peptidase -
  CKG21_RS04340 (CKG21_04340) coaE 847101..847736 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  CKG21_RS04345 (CKG21_04345) yacG 847718..847903 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  CKG21_RS04350 (CKG21_04350) holA 847904..848929 (-) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  CKG21_RS04355 (CKG21_04355) lptE 848972..849460 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=244452 CKG21_RS04320 WP_006248401.1 844261..844740(+) (pilA) [Mannheimia haemolytica strain USDA-ARS-USMARC-187]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=244452 CKG21_RS04320 WP_006248401.1 844261..844740(+) (pilA) [Mannheimia haemolytica strain USDA-ARS-USMARC-187]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491

Multiple sequence alignment