Detailed information NTG0243721

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CJ010_RS19175
• Genome accession: NZ_CP022958
• Coordinates: 4149385..4150695 (+)
• Length: 436 a.a.
• NCBI ID: WP_141019534.1
• Uniprot ID: A0A4Y6KW66
• Organism: Azoarcus sp. DD4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4144385..4155695

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CJ010_RS19155 (CJ010_19105)	-	4144489..4144983 (-)	495	WP_168224991.1	papain-like cysteine protease family protein	-
CJ010_RS19160 (CJ010_19110)	-	4145067..4146944 (-)	1878	WP_141019531.1	potassium transporter Kup	-
CJ010_RS19165 (CJ010_19115)	-	4146973..4147665 (-)	693	WP_141019532.1	response regulator	-
CJ010_RS19170 (CJ010_19120)	-	4147662..4149185 (-)	1524	WP_141019533.1	DUF4118 domain-containing protein	-
CJ010_RS19175 (CJ010_19125)	pilU	4149385..4150695 (+)	1311	WP_141019534.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CJ010_RS19180 (CJ010_19130)	-	4150704..4151378 (+)	675	WP_141019535.1	histidine phosphatase family protein	-
CJ010_RS19185 (CJ010_19135)	-	4151544..4152344 (+)	801	WP_141019536.1	CbbQ/NirQ/NorQ/GpvN family protein	-
CJ010_RS19190 (CJ010_19140)	-	4152361..4152765 (+)	405	WP_141019537.1	hypothetical protein	-
CJ010_RS19195 (CJ010_19145)	-	4152758..4153774 (+)	1017	WP_141019538.1	4Fe-4S binding protein	-

Sequence

Protein

• Length: 436 a.a.
• Molecular weight: 48774.66 Da
• Isoelectric Point: 6.2031

>NTDB_id=243721 CJ010_RS19175 WP_141019534.1 4149385..4150695(+) (pilU) [Azoarcus sp. DD4]
MTTFTEEQARAYMHKLLAAMSSAGGSDLFISHDFPPSMKAHGSMQPMTSQKLTGELTRSLAYSLMNAAQREEFEREMECN
FAISVPGVSRFRVNVFVQQTFVGMVIRTIAAEIPNFDKLGLPEVLKEVIMNKRGLVLLVGGTGSGKSTTLAAMIDYRNST
SAGHIITIEDPVEYVHKSKKSLITHREVGVDTHSWHHALKNTLRQAPDVILIGEIRDAETMEHAIAFAETGHLCLGTLHA
NNANQTIDRIINFFPEERRNQLLMDLSSNLRSIISQRLVKTADGKGRKAAIEILLNTSTIAERIFKGQYNDIKAIMEKSR
ELGMRTFDWALFELYNEGHITYEEAIRNADSVNELRLAIKLKSKRGEPSSASGLELTFDQEPTPEEVEAMREEEMQKQEA
RRQEIEQQQMLARLQPKPAPAEAPATPAPPAELSLE

Nucleotide

• Length: 1311 bp

>NTDB_id=243721 CJ010_RS19175 WP_141019534.1 4149385..4150695(+) (pilU) [Azoarcus sp. DD4]
ATGACCACGTTCACCGAAGAACAGGCACGTGCCTACATGCACAAGCTGCTGGCCGCGATGAGCAGCGCCGGCGGCTCCGA
CCTGTTCATCTCGCACGACTTCCCGCCCAGCATGAAGGCCCACGGCAGCATGCAGCCGATGACCTCGCAGAAGCTCACCG
GCGAGCTCACGCGCTCGCTGGCGTATTCGCTGATGAACGCAGCCCAGCGCGAGGAGTTCGAGCGCGAGATGGAGTGCAAC
TTCGCGATCAGCGTGCCGGGCGTGTCGCGCTTCCGGGTGAACGTGTTCGTGCAGCAGACCTTCGTCGGCATGGTGATCCG
CACCATCGCGGCCGAGATCCCGAACTTCGACAAGCTCGGCCTGCCCGAGGTGCTGAAGGAAGTCATCATGAACAAGCGCG
GGCTGGTGCTGCTGGTGGGCGGCACCGGCTCGGGCAAATCGACCACGCTGGCGGCGATGATCGACTACCGCAACAGCACC
TCGGCCGGGCACATCATCACCATCGAAGACCCGGTGGAGTACGTGCACAAGTCGAAGAAGTCGCTCATCACCCACCGCGA
GGTCGGCGTCGATACGCACTCCTGGCACCACGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCG
AGATCCGCGACGCCGAAACCATGGAGCACGCCATCGCCTTCGCCGAGACCGGGCACCTCTGCCTCGGCACCCTGCACGCC
AACAACGCCAACCAGACCATAGACCGCATCATCAACTTCTTCCCGGAGGAACGGCGCAACCAGCTGCTGATGGACCTCTC
CTCCAACCTGCGCAGCATCATCTCGCAGCGTCTGGTGAAGACGGCCGACGGCAAGGGCCGCAAAGCCGCGATCGAGATCC
TGCTCAATACCTCGACGATTGCCGAGCGCATCTTCAAGGGCCAGTACAACGACATCAAGGCCATCATGGAGAAGTCGCGC
GAGCTGGGCATGCGCACCTTCGACTGGGCACTGTTCGAGCTCTACAACGAAGGTCACATCACCTACGAGGAAGCGATCCG
CAACGCCGATTCGGTGAACGAGCTGCGCCTGGCGATCAAGCTCAAGAGCAAGCGCGGCGAGCCGAGCAGCGCCTCCGGCC
TGGAACTCACCTTCGACCAGGAACCGACGCCGGAAGAGGTCGAAGCGATGCGCGAAGAGGAAATGCAGAAGCAGGAAGCC
CGCCGCCAGGAAATCGAACAGCAGCAGATGCTGGCCCGCCTGCAGCCCAAGCCGGCCCCCGCCGAAGCGCCCGCCACGCC
GGCGCCGCCGGCCGAGCTGTCGCTCGAATAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (51-281)

3D structure

• AlphaFold DB: A0A4Y6KW66

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.989	85.321	0.486
pilU	Acinetobacter baylyi ADP1	52.825	81.193	0.429
pilU	Vibrio cholerae strain A1552	47.934	83.257	0.399