Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CGP94_RS08295 Genome accession   NZ_CP022898
Coordinates   1654634..1655320 (-) Length   228 a.a.
NCBI ID   WP_072357801.1    Uniprot ID   A0A6B5EZE0
Organism   Staphylococcus aureus strain 422     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1649634..1660320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGP94_RS08275 (CGP94_01525) rpsT 1650548..1650799 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  CGP94_RS08280 (CGP94_01526) holA 1650844..1651818 (-) 975 WP_001282559.1 DNA polymerase III subunit delta -
  CGP94_RS08285 (CGP94_01527) comEC 1651875..1654076 (-) 2202 WP_042908045.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  CGP94_RS08290 (CGP94_01528) - 1654081..1654542 (-) 462 WP_000439693.1 ComE operon protein 2 -
  CGP94_RS08295 (CGP94_01529) comEA 1654634..1655320 (-) 687 WP_072357801.1 ComEA family DNA-binding protein Machinery gene
  CGP94_RS08300 (CGP94_01530) - 1655360..1656076 (-) 717 WP_000084828.1 class I SAM-dependent methyltransferase -
  CGP94_RS08305 (CGP94_01531) rsfS 1656079..1656432 (-) 354 WP_001088020.1 ribosome silencing factor -
  CGP94_RS08310 (CGP94_01532) yqeK 1656433..1657017 (-) 585 WP_001019325.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  CGP94_RS08315 (CGP94_01533) nadD 1657007..1657576 (-) 570 WP_000725160.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  CGP94_RS08320 (CGP94_01534) yhbY 1657579..1657869 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  CGP94_RS08325 (CGP94_01535) aroE 1657873..1658679 (-) 807 WP_000666752.1 shikimate dehydrogenase -
  CGP94_RS08330 (CGP94_01536) yqeH 1658693..1659793 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25759.47 Da        Isoelectric Point: 9.6753

>NTDB_id=242950 CGP94_RS08295 WP_072357801.1 1654634..1655320(-) (comEA) [Staphylococcus aureus strain 422]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKLEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLSDQKMIFIPHKGQKNVEPQIGVNSVHVKN
GNINNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=242950 CGP94_RS08295 WP_072357801.1 1654634..1655320(-) (comEA) [Staphylococcus aureus strain 422]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTATCAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGGAGTAAACAGTGTGCACGTAAAAAAT
GGAAACATAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6B5EZE0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

97.797

99.561

0.974
  comEA Staphylococcus aureus N315

97.797

99.561

0.974

Multiple sequence alignment