Detailed information    

insolico Bioinformatically predicted

Overview


Name   phrF   Type   Regulator
Locus tag   CJZ71_RS10225 Genome accession   NZ_CP022891
Coordinates   1882659..1882778 (+) Length   39 a.a.
NCBI ID   WP_009968329.1    Uniprot ID   A0A199WEM2
Organism   Bacillus subtilis strain DKU_NT_03     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context


Location: 1877659..1887778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJZ71_RS10200 (CJZ71_10200) albG 1877827..1878528 (+) 702 WP_069837830.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  CJZ71_RS10205 (CJZ71_10205) ywhL 1878534..1879910 (-) 1377 WP_069837831.1 YncE family protein -
  CJZ71_RS10210 (CJZ71_10210) ywhK 1879949..1881300 (-) 1352 Protein_2017 YncE family protein -
  CJZ71_RS23165 (CJZ71_10215) - 1881297..1881527 (-) 231 Protein_2018 hypothetical protein -
  CJZ71_RS10220 (CJZ71_10220) rapF 1881530..1882675 (+) 1146 WP_069837832.1 response regulator aspartate phosphatase RapF Regulator
  CJZ71_RS10225 (CJZ71_10225) phrF 1882659..1882778 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  CJZ71_RS10230 (CJZ71_10230) - 1882876..1883303 (+) 428 Protein_2021 YbaK/EbsC family protein -
  CJZ71_RS10235 (CJZ71_10235) speB 1883368..1884240 (-) 873 WP_003227545.1 agmatinase -
  CJZ71_RS10240 (CJZ71_10240) speE 1884301..1885131 (-) 831 WP_003227543.1 spermidine synthase -
  CJZ71_RS10245 (CJZ71_10245) pbpG 1885333..1887408 (+) 2076 WP_069837833.1 transglycosylase domain-containing protein -

Sequence


Protein


Download         Length: 39 a.a.        Molecular weight: 4199.10 Da        Isoelectric Point: 10.4997

>NTDB_id=242638 CJZ71_RS10225 WP_009968329.1 1882659..1882778(+) (phrF) [Bacillus subtilis strain DKU_NT_03]
MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=242638 CJZ71_RS10225 WP_009968329.1 1882659..1882778(+) (phrF) [Bacillus subtilis strain DKU_NT_03]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACC
TACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A199WEM2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

100

100

1

  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487


Multiple sequence alignment