Detailed information NTG0241339

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CHN56_RS18635
• Genome accession: NZ_CP022654
• Coordinates: 3654118..3654732 (+)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain SCDB 291
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3649118..3659732

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CHN56_RS18600 (CHN56_03683)	aroE	3649805..3650647 (+)	843	WP_094247716.1	shikimate dehydrogenase	-
CHN56_RS18605 (CHN56_03684)	yhbY	3650641..3650931 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
CHN56_RS18610 (CHN56_03685)	-	3650942..3651511 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
CHN56_RS18615 (CHN56_03686)	yqeK	3651501..3652061 (+)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CHN56_RS18620 (CHN56_03687)	rsfS	3652079..3652435 (+)	357	WP_003152864.1	ribosome silencing factor	-
CHN56_RS18625 (CHN56_03688)	-	3652432..3653169 (+)	738	WP_031378935.1	class I SAM-dependent methyltransferase	-
CHN56_RS18630 (CHN56_03689)	comER	3653238..3654059 (-)	822	WP_014305449.1	late competence protein ComER	-
CHN56_RS18635 (CHN56_03690)	comEA	3654118..3654732 (+)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
CHN56_RS18640 (CHN56_03691)	-	3654799..3655368 (+)	570	WP_003152868.1	ComE operon protein 2	-
CHN56_RS18645 (CHN56_03692)	comEC	3655369..3657720 (+)	2352	WP_094247717.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CHN56_RS18650	-	3657739..3657873 (-)	135	WP_003152870.1	YqzM family protein	-
CHN56_RS21420	-	3657914..3658068 (+)	155	Protein_3585	hypothetical protein	-
CHN56_RS18655 (CHN56_03693)	holA	3658108..3659149 (+)	1042	Protein_3586	DNA polymerase III subunit delta	-
CHN56_RS18660 (CHN56_03694)	rpsT	3659166..3659432 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=241339 CHN56_RS18635 WP_013352942.1 3654118..3654732(+) (comEA) [Bacillus velezensis strain SCDB 291]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=241339 CHN56_RS18635 WP_013352942.1 3654118..3654732(+) (comEA) [Bacillus velezensis strain SCDB 291]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363