Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BaGK_RS13275 Genome accession   NZ_CP022653
Coordinates   2725527..2726597 (-) Length   356 a.a.
NCBI ID   WP_088117573.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain GQJK17     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2720527..2731597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BaGK_RS13230 (BaGK_13230) tapA 2721062..2721763 (-) 702 WP_094232413.1 amyloid fiber anchoring/assembly protein TapA -
  BaGK_RS13235 (BaGK_13235) - 2722025..2722348 (+) 324 WP_003325436.1 DUF3889 domain-containing protein -
  BaGK_RS13240 (BaGK_13240) - 2722395..2722574 (-) 180 WP_003325435.1 YqzE family protein -
  BaGK_RS13245 (BaGK_13245) comGG 2722644..2723018 (-) 375 WP_088117569.1 competence type IV pilus minor pilin ComGG Machinery gene
  BaGK_RS13250 (BaGK_13250) comGF 2723019..2723414 (-) 396 WP_309484978.1 competence type IV pilus minor pilin ComGF Machinery gene
  BaGK_RS13255 (BaGK_13255) comGE 2723428..2723775 (-) 348 WP_094232414.1 competence type IV pilus minor pilin ComGE Machinery gene
  BaGK_RS13260 (BaGK_13260) comGD 2723759..2724199 (-) 441 WP_094232415.1 competence type IV pilus minor pilin ComGD Machinery gene
  BaGK_RS13265 (BaGK_13265) comGC 2724189..2724455 (-) 267 WP_047937532.1 competence type IV pilus major pilin ComGC Machinery gene
  BaGK_RS13270 (BaGK_13270) comGB 2724503..2725540 (-) 1038 WP_088117572.1 competence type IV pilus assembly protein ComGB Machinery gene
  BaGK_RS13275 (BaGK_13275) comGA 2725527..2726597 (-) 1071 WP_088117573.1 competence type IV pilus ATPase ComGA Machinery gene
  BaGK_RS13280 (BaGK_13280) - 2726750..2726974 (-) 225 WP_157729161.1 hypothetical protein -
  BaGK_RS13285 (BaGK_13285) - 2726977..2727930 (-) 954 WP_088117575.1 magnesium transporter CorA family protein -
  BaGK_RS13290 (BaGK_13290) - 2728052..2729400 (+) 1349 Protein_2608 hemolysin family protein -
  BaGK_RS13295 (BaGK_13295) - 2729467..2730318 (-) 852 WP_088117577.1 STAS domain-containing protein -
  BaGK_RS13305 (BaGK_13305) - 2730585..2730965 (-) 381 WP_003325422.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BaGK_RS13310 (BaGK_13310) - 2731203..2731448 (+) 246 WP_003325421.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40453.77 Da        Isoelectric Point: 9.4624

>NTDB_id=241247 BaGK_RS13275 WP_088117573.1 2725527..2726597(-) (comGA) [Bacillus atrophaeus strain GQJK17]
MDPIEKTSRTLIEEAYSVKASDIHIVPREQDALIHFRIDHALLKKRTMKKEECVRLISHFKFLSAMDIGERRKPQSGSLS
LKLKKGTVYLRMSTLPTINDESLVIRVLPQHNIPAIERLSLFPKTGATLLSFLKHSHGLIIFTGPTGSGKSTTLYSLVQY
AKKHFNRNIITLEDPVETRDQDVLQVQVNEKAGITYSAGLKAILRHDPDMIVLGEIRDAETAKIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGVNMNEIEQTAIAIAAQRLVDLTCPFCENGCSSVYCRITRDVRRATIYELLYGKNLQQCIQEARGE
RANYQYRTLRQIIRKGIALGYLTTNNYDRWVYHEED

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=241247 BaGK_RS13275 WP_088117573.1 2725527..2726597(-) (comGA) [Bacillus atrophaeus strain GQJK17]
TTGGATCCGATAGAAAAAACTAGCAGGACATTAATTGAAGAGGCATATTCCGTGAAGGCCTCGGATATTCACATTGTACC
AAGGGAGCAAGATGCTTTGATCCATTTTCGGATTGACCATGCGTTGCTGAAGAAGCGGACAATGAAAAAGGAAGAGTGCG
TTCGGCTCATTTCCCATTTTAAATTTCTGTCAGCCATGGATATAGGAGAAAGGCGGAAGCCGCAAAGTGGTTCACTTTCT
TTAAAGCTTAAAAAAGGGACAGTTTATCTCAGAATGTCGACACTTCCTACGATTAATGATGAAAGCCTCGTGATCAGAGT
TTTGCCTCAGCATAATATTCCTGCGATCGAAAGACTGTCCCTGTTCCCAAAGACAGGCGCCACGCTGCTATCCTTTTTAA
AGCACTCCCATGGCCTCATTATTTTTACCGGTCCGACTGGCTCAGGGAAATCTACCACCCTATATTCACTGGTTCAATAT
GCAAAAAAGCATTTCAACCGAAATATTATCACTCTCGAAGATCCTGTGGAAACTAGAGATCAGGATGTCTTGCAGGTACA
GGTGAATGAAAAAGCCGGCATTACGTACTCAGCGGGCCTGAAGGCGATTTTGCGCCATGATCCAGACATGATTGTTCTGG
GGGAAATCAGGGATGCTGAAACAGCAAAAATCGCCGTGCGGGCTGCTATGACAGGCCATTTGGTATTAACAAGCCTGCAC
ACAAGAGATGCCAAGGGCGCCATTTACAGACTGCTTGAATTCGGAGTAAATATGAATGAAATAGAGCAAACAGCGATTGC
GATAGCAGCCCAGCGATTAGTCGACTTAACCTGTCCATTTTGTGAAAACGGCTGTTCCTCTGTTTATTGCCGGATTACAC
GTGATGTAAGGCGAGCGACCATTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATACAAGAAGCTAGAGGAGAA
CGGGCCAATTATCAGTACCGAACCTTACGGCAGATTATCAGAAAAGGAATCGCGCTTGGTTATCTAACGACGAATAATTA
CGACCGGTGGGTTTATCATGAAGAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

87.921

100

0.879
  pilB Glaesserella parasuis strain SC1401

44.027

82.303

0.362

Multiple sequence alignment