Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   BaGK_RS13270 Genome accession   NZ_CP022653
Coordinates   2724503..2725540 (-) Length   345 a.a.
NCBI ID   WP_088117572.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain GQJK17     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2719503..2730540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BaGK_RS13220 (BaGK_13220) tasA 2719660..2720442 (-) 783 WP_063638541.1 biofilm matrix protein TasA -
  BaGK_RS13225 (BaGK_13225) sipW 2720506..2721078 (-) 573 WP_094233201.1 signal peptidase I SipW -
  BaGK_RS13230 (BaGK_13230) tapA 2721062..2721763 (-) 702 WP_094232413.1 amyloid fiber anchoring/assembly protein TapA -
  BaGK_RS13235 (BaGK_13235) - 2722025..2722348 (+) 324 WP_003325436.1 DUF3889 domain-containing protein -
  BaGK_RS13240 (BaGK_13240) - 2722395..2722574 (-) 180 WP_003325435.1 YqzE family protein -
  BaGK_RS13245 (BaGK_13245) comGG 2722644..2723018 (-) 375 WP_088117569.1 competence type IV pilus minor pilin ComGG Machinery gene
  BaGK_RS13250 (BaGK_13250) comGF 2723019..2723414 (-) 396 WP_309484978.1 competence type IV pilus minor pilin ComGF Machinery gene
  BaGK_RS13255 (BaGK_13255) comGE 2723428..2723775 (-) 348 WP_094232414.1 competence type IV pilus minor pilin ComGE Machinery gene
  BaGK_RS13260 (BaGK_13260) comGD 2723759..2724199 (-) 441 WP_094232415.1 competence type IV pilus minor pilin ComGD Machinery gene
  BaGK_RS13265 (BaGK_13265) comGC 2724189..2724455 (-) 267 WP_047937532.1 competence type IV pilus major pilin ComGC Machinery gene
  BaGK_RS13270 (BaGK_13270) comGB 2724503..2725540 (-) 1038 WP_088117572.1 competence type IV pilus assembly protein ComGB Machinery gene
  BaGK_RS13275 (BaGK_13275) comGA 2725527..2726597 (-) 1071 WP_088117573.1 competence type IV pilus ATPase ComGA Machinery gene
  BaGK_RS13280 (BaGK_13280) - 2726750..2726974 (-) 225 WP_157729161.1 hypothetical protein -
  BaGK_RS13285 (BaGK_13285) - 2726977..2727930 (-) 954 WP_088117575.1 magnesium transporter CorA family protein -
  BaGK_RS13290 (BaGK_13290) - 2728052..2729400 (+) 1349 Protein_2608 hemolysin family protein -
  BaGK_RS13295 (BaGK_13295) - 2729467..2730318 (-) 852 WP_088117577.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 40112.30 Da        Isoelectric Point: 9.6748

>NTDB_id=241246 BaGK_RS13270 WP_088117572.1 2724503..2725540(-) (comGB) [Bacillus atrophaeus strain GQJK17]
MKRIKKSWPINDQANLLNRLGDMTENGYNLLDGLRLMELQMNKRQRTEMSAAVNRLREGEPFYQVLQALAFHKEAVSICY
LAEMHGELSVAMRQSGGLLERKLTQANQLKKVIRYPIFLIFTVFVMFYILQAVIIPQFSGVYQSMNMETSRTTAVIFAFF
QHVHYAVIGFVLLALGAALYYWFGFKKKSPADKMLFFVRIPILGRLVILFNSYFFSLQLSSLLKSGLSIYDSLKAFKEQS
FLPFYRQEAEQIINQLRAGESIDRALYKHPCYENDFCEVVLHGQLSGQLHRELFTYSQFLMERLERKVEKWTGILQPVIY
GIVAGMILIVYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=241246 BaGK_RS13270 WP_088117572.1 2724503..2725540(-) (comGB) [Bacillus atrophaeus strain GQJK17]
ATGAAGAGGATTAAAAAAAGCTGGCCGATAAACGATCAAGCTAATTTGCTAAACAGACTCGGTGACATGACAGAAAACGG
ATACAATCTTCTGGATGGATTGCGGTTAATGGAACTGCAAATGAATAAGCGTCAGCGTACGGAAATGTCTGCTGCTGTAA
ATCGTTTACGAGAGGGGGAACCATTCTATCAGGTTTTGCAGGCTTTGGCTTTCCACAAGGAAGCCGTCAGCATTTGTTAT
TTAGCAGAAATGCATGGAGAATTGTCCGTTGCTATGAGGCAAAGCGGTGGGCTGTTGGAAAGGAAATTAACACAGGCCAA
TCAGCTGAAAAAAGTCATCCGTTATCCGATTTTCCTGATCTTCACTGTCTTTGTGATGTTTTATATCCTGCAAGCTGTCA
TCATCCCGCAATTTTCAGGAGTTTATCAGTCCATGAATATGGAAACCTCACGCACAACCGCTGTTATATTCGCTTTTTTT
CAGCACGTTCATTATGCAGTGATTGGTTTTGTCTTGCTTGCTCTCGGAGCGGCGCTCTATTACTGGTTTGGATTTAAGAA
GAAGTCCCCGGCAGACAAAATGCTCTTTTTTGTACGGATACCCATACTCGGAAGACTTGTCATTTTATTTAACAGCTACT
TTTTTTCTTTGCAGCTCAGCAGCCTATTAAAGTCTGGACTGTCTATTTACGACAGCCTTAAGGCCTTTAAAGAGCAATCG
TTCCTCCCTTTTTACCGTCAGGAGGCAGAACAAATCATTAACCAATTAAGGGCTGGAGAGTCCATAGATAGAGCTCTGTA
TAAACACCCATGCTATGAAAATGACTTTTGTGAAGTGGTGTTGCACGGCCAATTAAGCGGGCAGCTGCATCGTGAACTAT
TCACCTACAGCCAGTTTTTAATGGAGCGGCTGGAGCGAAAGGTTGAAAAATGGACGGGAATCCTTCAGCCCGTCATCTAC
GGTATTGTTGCCGGGATGATTTTAATTGTGTATTTGTCGATGCTTTTGCCTATGTATCAGATGATGAATCAATTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

69.35

93.623

0.649

Multiple sequence alignment