Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   Sp14A_RS08655 Genome accession   NZ_CP022601
Coordinates   1684850..1686151 (-) Length   433 a.a.
NCBI ID   WP_115130707.1    Uniprot ID   A0A345VLT2
Organism   Streptococcus pluranimalium strain 14A0014     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1679850..1691151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Sp14A_RS08645 (Sp14A_17890) raiA 1683562..1684110 (-) 549 WP_115130705.1 ribosome-associated translation inhibitor RaiA -
  Sp14A_RS08650 (Sp14A_17900) - 1684191..1684853 (-) 663 WP_115130706.1 ComF family protein -
  Sp14A_RS08655 (Sp14A_17910) comFA/cflA 1684850..1686151 (-) 1302 WP_115130707.1 DEAD/DEAH box helicase Machinery gene
  Sp14A_RS08660 (Sp14A_17920) - 1686198..1686827 (+) 630 WP_115130708.1 YigZ family protein -
  Sp14A_RS08665 (Sp14A_17930) cysK 1686925..1687854 (+) 930 WP_115130709.1 cysteine synthase A -
  Sp14A_RS08675 tnpA 1688007..1688480 (-) 474 Protein_1658 IS200/IS605 family transposase -
  Sp14A_RS08680 (Sp14A_17950) - 1688713..1689072 (-) 360 WP_104968357.1 S1 RNA-binding domain-containing protein -
  Sp14A_RS08685 (Sp14A_17960) - 1689072..1690472 (-) 1401 WP_115130710.1 Cof-type HAD-IIB family hydrolase -

Sequence

Protein


Download         Length: 433 a.a.        Molecular weight: 49203.96 Da        Isoelectric Point: 9.4151

>NTDB_id=241068 Sp14A_RS08655 WP_115130707.1 1684850..1686151(-) (comFA/cflA) [Streptococcus pluranimalium strain 14A0014]
MKTLKEKSGCWFAGSSITAEEREEAIKIPAFTQENGNLTCNRCSEKITTDWQLPDGTRYCRSCILLGRLTEDDHLYYFPP
KTFPKRNALQWKGKLTSYQKEISGSLVQSLEDRKDSLVHAVTGAGKTEMIYEVVAQVIDEGGQVCLASPRIDVCIELYKR
LSKDFSCDIDLLHGDSEPYSGSPLIIATTHQLLKFYHSFDLILVDEVDAFPYVDNKILYHAVRTAKKADGLTIYMTATST
EELDKQVKKGILKKRTLSRRFHGNPLIEPRNIWLTGLEKKVDSGKIPLKLKRYLERQFQTGYPLLIFVPDIAFGKKIKKV
LCQQYPKVSIGFVSSNSPDRLEEVEMFRHGDLRVLISTTILERGVTFPKVDVFVLLAHHKLFTSSSLIQIGGRVGRSLER
PTGDLFFFHEGLTRAIKKTKKEIRQMNNLGGFK

Nucleotide


Download         Length: 1302 bp        

>NTDB_id=241068 Sp14A_RS08655 WP_115130707.1 1684850..1686151(-) (comFA/cflA) [Streptococcus pluranimalium strain 14A0014]
ATGAAAACGCTAAAAGAAAAAAGTGGTTGCTGGTTTGCAGGTTCTAGTATCACGGCAGAGGAAAGAGAAGAAGCCATAAA
GATACCTGCTTTCACTCAAGAAAATGGGAATCTTACTTGTAATAGATGTTCAGAAAAGATTACAACTGATTGGCAGTTGC
CGGATGGGACACGTTATTGTCGCTCCTGTATTTTATTAGGTAGATTGACGGAAGATGACCATCTCTATTATTTTCCACCG
AAAACTTTCCCAAAAAGAAATGCTTTACAATGGAAAGGCAAATTAACTAGTTATCAAAAAGAAATTTCAGGTAGCCTTGT
ACAATCACTTGAGGATAGAAAAGATAGTTTGGTACATGCCGTAACAGGAGCTGGAAAAACGGAAATGATCTATGAAGTAG
TCGCTCAAGTGATTGATGAAGGTGGACAAGTTTGTTTAGCCAGTCCTCGTATAGATGTTTGTATTGAATTATACAAGCGA
CTCAGCAAGGATTTTTCTTGTGATATTGATTTGTTACATGGTGATTCTGAACCATATAGTGGCTCCCCTTTGATTATTGC
AACTACCCATCAGCTGTTAAAGTTTTACCACTCTTTTGATCTTATATTAGTTGATGAAGTAGATGCCTTTCCCTACGTCG
ATAATAAAATACTTTATCATGCTGTTAGAACAGCGAAAAAAGCAGATGGTCTAACCATCTATATGACAGCAACTTCGACA
GAAGAATTAGATAAACAAGTCAAAAAAGGAATCTTAAAAAAACGAACTTTATCGCGAAGATTTCATGGGAATCCCTTAAT
TGAACCACGGAATATTTGGCTAACTGGACTGGAGAAAAAGGTTGACTCTGGTAAGATACCATTGAAACTAAAACGATATC
TAGAGAGGCAATTTCAAACAGGCTACCCGCTTTTAATTTTTGTACCAGATATTGCTTTTGGAAAGAAAATAAAGAAAGTT
CTTTGTCAACAATATCCCAAAGTTTCTATAGGTTTTGTTTCTAGTAATAGTCCAGATAGATTAGAAGAAGTAGAAATGTT
CAGACATGGTGATTTAAGAGTTCTCATATCAACGACAATTTTAGAAAGAGGAGTGACATTTCCTAAAGTGGATGTCTTTG
TTTTACTAGCTCATCATAAATTATTTACTAGCTCTAGTCTTATTCAGATAGGTGGTCGAGTAGGACGGTCATTAGAGAGA
CCGACAGGAGATTTATTCTTCTTTCATGAAGGGCTTACAAGGGCTATCAAAAAGACTAAAAAAGAAATTCGACAGATGAA
CAATTTGGGAGGCTTCAAGTGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(297-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A345VLT2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

58.796

99.769

0.587
  comFA/cflA Streptococcus pneumoniae D39

58.796

99.769

0.587
  comFA/cflA Streptococcus pneumoniae R6

58.796

99.769

0.587
  comFA/cflA Streptococcus pneumoniae TIGR4

58.796

99.769

0.587
  comFA/cflA Streptococcus mitis NCTC 12261

58.333

99.769

0.582
  comFA/cflA Streptococcus mitis SK321

57.407

99.769

0.573
  comFA Lactococcus lactis subsp. cremoris KW2

47.319

99.076

0.469
  comFA Bacillus subtilis subsp. subtilis str. 168

37.209

99.307

0.37
  comFA Latilactobacillus sakei subsp. sakei 23K

38.916

93.764

0.365

Multiple sequence alignment