Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CHR53_RS19715 Genome accession   NZ_CP022572
Coordinates   4025359..4026441 (-) Length   360 a.a.
NCBI ID   WP_066385505.1    Uniprot ID   A0A3Q9QWG4
Organism   Neobacillus mesonae strain H20-5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4020359..4031441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHR53_RS19670 (CHR53_19755) - 4020927..4021169 (-) 243 WP_066385429.1 DUF2626 domain-containing protein -
  CHR53_RS19675 (CHR53_19760) - 4021254..4021949 (-) 696 WP_066385431.1 helix-turn-helix domain-containing protein -
  CHR53_RS19680 (CHR53_19765) - 4022168..4022353 (-) 186 WP_127488059.1 YqzE family protein -
  CHR53_RS19685 (CHR53_19770) comGG 4022416..4022799 (-) 384 WP_066385435.1 competence type IV pilus minor pilin ComGG -
  CHR53_RS19690 (CHR53_19775) comGF 4022796..4023275 (-) 480 WP_127489986.1 competence type IV pilus minor pilin ComGF -
  CHR53_RS19695 (CHR53_19780) - 4023226..4023564 (-) 339 WP_066385437.1 hypothetical protein -
  CHR53_RS19700 (CHR53_19785) comGD 4023548..4023988 (-) 441 WP_066385439.1 competence type IV pilus minor pilin ComGD -
  CHR53_RS19705 (CHR53_19790) comGC 4023988..4024320 (-) 333 WP_066385444.1 competence type IV pilus major pilin ComGC Machinery gene
  CHR53_RS19710 (CHR53_19795) comGB 4024341..4025372 (-) 1032 WP_127488060.1 competence type IV pilus assembly protein ComGB -
  CHR53_RS19715 (CHR53_19800) comGA 4025359..4026441 (-) 1083 WP_066385505.1 competence type IV pilus ATPase ComGA Machinery gene
  CHR53_RS19720 (CHR53_19805) - 4026568..4027545 (-) 978 WP_127488061.1 ROK family glucokinase -
  CHR53_RS19725 (CHR53_19810) - 4027552..4027761 (-) 210 WP_066385506.1 YqgQ family protein -
  CHR53_RS19730 (CHR53_19815) - 4027960..4028787 (+) 828 WP_127488062.1 hypothetical protein -
  CHR53_RS19735 (CHR53_19820) - 4028750..4030312 (-) 1563 WP_066400759.1 rhomboid family intramembrane serine protease -
  CHR53_RS19740 (CHR53_19825) - 4030422..4030613 (-) 192 WP_066385508.1 hypothetical protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40377.64 Da        Isoelectric Point: 7.7492

>NTDB_id=240885 CHR53_RS19715 WP_066385505.1 4025359..4026441(-) (comGA) [Neobacillus mesonae strain H20-5]
MFPIVSEIEILANRIITDAARNQATDIHIIPRKNDTLVQIRLTNKLIPRLSLPKDECDRLISHFKFTANMDIGERRRPQS
GAIVCDVNGQLMGLRLSTLPSNNRESLVIRLLPQQDQIPFHQLSLFPSMTRKLLALLKHAHGLIIFTGPTGSGKTTTLYS
LLNETAHLFHRNVITLEDPVEKNYDSVLQVQVNEKAGVTYAAGLKAILRHDPDIIMVGEIRDAETAKIAVRAALTGHLVL
STMHTRDAKGAVFRLEEFGVNWLEVEQTLIAVTAQRLVELTCPFCEGECPPHCYSGGRWKRASVFELLSGRNLSAIMRAA
MGEKIDTNYRTLKDVIRKGIALGYIQSSEYDRLVYDDEET

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=240885 CHR53_RS19715 WP_066385505.1 4025359..4026441(-) (comGA) [Neobacillus mesonae strain H20-5]
GTGTTTCCAATTGTTAGTGAAATTGAAATTTTAGCTAACAGAATTATTACTGATGCGGCAAGAAACCAGGCGACAGATAT
TCACATTATTCCTCGTAAGAATGACACCCTAGTGCAAATTAGGTTAACAAACAAACTGATTCCTCGATTATCACTTCCGA
AAGATGAATGCGACAGATTGATTTCCCATTTTAAATTTACAGCAAATATGGATATCGGAGAACGCAGACGCCCGCAAAGC
GGTGCGATTGTTTGTGATGTTAACGGACAACTAATGGGTCTAAGGCTGTCTACCCTTCCTTCCAACAACAGAGAAAGCCT
TGTCATTCGCCTATTGCCGCAGCAAGATCAAATTCCTTTTCACCAGCTCTCACTCTTTCCATCGATGACACGAAAATTAT
TAGCTTTGCTCAAACATGCACATGGTTTAATTATTTTCACTGGGCCAACTGGGTCTGGGAAAACCACGACCTTGTATTCA
CTTTTAAACGAAACTGCACACCTGTTCCATCGCAATGTGATTACTCTTGAAGACCCAGTTGAAAAAAATTATGATTCGGT
CCTCCAGGTTCAGGTGAATGAAAAAGCGGGGGTGACTTATGCTGCAGGTTTAAAAGCAATTCTTCGACATGATCCGGATA
TTATAATGGTCGGGGAAATCAGGGACGCGGAAACTGCAAAAATTGCCGTCCGCGCAGCTTTAACGGGTCATTTAGTTTTA
AGCACAATGCATACCCGCGATGCGAAAGGTGCGGTCTTTCGTTTGGAGGAGTTTGGCGTAAACTGGTTAGAGGTGGAACA
AACTTTAATTGCCGTCACCGCACAAAGATTAGTTGAATTAACCTGCCCATTTTGTGAAGGCGAATGCCCACCGCATTGTT
ATTCAGGCGGCAGGTGGAAAAGAGCAAGTGTGTTTGAACTGTTATCGGGAAGAAATTTATCTGCCATAATGAGAGCGGCA
ATGGGAGAAAAGATTGACACAAATTATCGGACATTAAAAGATGTGATTAGAAAAGGGATTGCGCTAGGCTATATTCAATC
CTCTGAATATGACCGGCTGGTGTATGATGATGAAGAAACATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q9QWG4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.899

98.889

0.553
  pilB Glaesserella parasuis strain SC1401

39.548

98.333

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.533

100

0.381

Multiple sequence alignment