Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   C7M26_RS00860 Genome accession   NZ_CP028212
Coordinates   131910..132527 (+) Length   205 a.a.
NCBI ID   WP_032722140.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM102748     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 126910..137527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M26_RS00825 (C7M26_00156) aroE 127568..128410 (+) 843 WP_003229967.1 shikimate dehydrogenase -
  C7M26_RS00830 (C7M26_00157) yhbY 128404..128694 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  C7M26_RS00835 (C7M26_00158) nadD 128706..129275 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  C7M26_RS00840 (C7M26_00159) yqeK 129265..129825 (+) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  C7M26_RS00845 (C7M26_00160) rsfS 129843..130199 (+) 357 WP_014480315.1 ribosome silencing factor -
  C7M26_RS00850 (C7M26_00161) yqeM 130196..130939 (+) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  C7M26_RS00855 (C7M26_00162) comER 131005..131826 (-) 822 WP_032722142.1 late competence protein ComER -
  C7M26_RS00860 (C7M26_00163) comEA 131910..132527 (+) 618 WP_032722140.1 competence protein ComEA Machinery gene
  C7M26_RS00865 (C7M26_00164) comEB 132594..133163 (+) 570 WP_003229978.1 ComE operon protein 2 -
  C7M26_RS00870 (C7M26_00165) comEC 133167..135497 (+) 2331 WP_160214965.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  C7M26_RS00875 (C7M26_00166) yqzM 135537..135671 (-) 135 WP_003229983.1 YqzM family protein -
  C7M26_RS00880 - 135712..135861 (+) 150 WP_003229985.1 hypothetical protein -
  C7M26_RS00885 (C7M26_00167) holA 135901..136944 (+) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  C7M26_RS00890 (C7M26_00168) rpsT 136959..137225 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21748.46 Da        Isoelectric Point: 4.8354

>NTDB_id=239838 C7M26_RS00860 WP_032722140.1 131910..132527(+) (comEA) [Bacillus subtilis strain SRCM102748]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVRHPGVYELRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFPTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=239838 C7M26_RS00860 WP_032722140.1 131910..132527(+) (comEA) [Bacillus subtilis strain SRCM102748]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAATGAAACAATTGTGATAGACATCAAAGGTGCTGTTCGGCATCCTGGCGTTTATGAATTGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCCAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.537

100

0.985
  comEA Staphylococcus aureus MW2

36.364

100

0.39
  comEA Staphylococcus aureus N315

35.455

100

0.38

Multiple sequence alignment