Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C7A99_RS06215 Genome accession   NZ_CP028192
Coordinates   1200380..1201039 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain CFSAN018748     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1195380..1206039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7A99_RS06190 (C7A99_06180) - 1196122..1197069 (+) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -
  C7A99_RS06195 (C7A99_06185) - 1197066..1197953 (+) 888 WP_107235620.1 MurR/RpiR family transcriptional regulator -
  C7A99_RS06200 (C7A99_06190) ygiN 1197998..1198312 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  C7A99_RS06205 (C7A99_06195) mdaB 1198343..1198924 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C7A99_RS06210 (C7A99_06200) qseC 1199034..1200383 (-) 1350 WP_000673373.1 quorum sensing histidine kinase QseC -
  C7A99_RS06215 (C7A99_06205) ciaR 1200380..1201039 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  C7A99_RS06220 (C7A99_06210) ygiW 1201191..1201583 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C7A99_RS06225 (C7A99_06215) ygiV 1201636..1202118 (+) 483 WP_000183492.1 GyrI-like domain-containing protein -
  C7A99_RS06230 (C7A99_06220) ygiS 1202227..1203834 (+) 1608 WP_063085111.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=239327 C7A99_RS06215 WP_001221493.1 1200380..1201039(-) (ciaR) [Escherichia coli strain CFSAN018748]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=239327 C7A99_RS06215 WP_001221493.1 1200380..1201039(-) (ciaR) [Escherichia coli strain CFSAN018748]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment