Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CFN60_RS12035 Genome accession   NZ_CP022341
Coordinates   2474647..2475717 (-) Length   356 a.a.
NCBI ID   WP_014418378.1    Uniprot ID   A0AAP4DGV9
Organism   Bacillus velezensis strain 157     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2469647..2480717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFN60_RS11985 (CFN60_11980) sipW 2469706..2470290 (-) 585 WP_022552967.1 signal peptidase I SipW -
  CFN60_RS11990 (CFN60_11985) tapA 2470262..2470933 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  CFN60_RS11995 (CFN60_11990) - 2471192..2471521 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  CFN60_RS12000 (CFN60_11995) - 2471562..2471741 (-) 180 WP_022552966.1 YqzE family protein -
  CFN60_RS12005 (CFN60_12000) comGG 2471798..2472175 (-) 378 WP_022552965.1 competence type IV pilus minor pilin ComGG Machinery gene
  CFN60_RS12010 (CFN60_12005) comGF 2472176..2472571 (-) 396 WP_046559876.1 competence type IV pilus minor pilin ComGF -
  CFN60_RS12015 (CFN60_12010) comGE 2472585..2472899 (-) 315 WP_021494312.1 competence type IV pilus minor pilin ComGE Machinery gene
  CFN60_RS12020 (CFN60_12015) comGD 2472883..2473320 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  CFN60_RS12025 (CFN60_12020) comGC 2473310..2473618 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  CFN60_RS12030 (CFN60_12025) comGB 2473623..2474660 (-) 1038 WP_022552962.1 competence type IV pilus assembly protein ComGB Machinery gene
  CFN60_RS12035 (CFN60_12030) comGA 2474647..2475717 (-) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  CFN60_RS12040 (CFN60_12035) - 2475910..2476860 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  CFN60_RS12045 (CFN60_12040) - 2477006..2478307 (+) 1302 WP_022552961.1 hemolysin family protein -
  CFN60_RS12050 (CFN60_12045) - 2478345..2479175 (-) 831 WP_022552960.1 STAS domain-containing protein -
  CFN60_RS12060 (CFN60_12055) - 2479382..2479762 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  CFN60_RS12065 (CFN60_12060) - 2479999..2480244 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=238460 CFN60_RS12035 WP_014418378.1 2474647..2475717(-) (comGA) [Bacillus velezensis strain 157]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=238460 CFN60_RS12035 WP_014418378.1 2474647..2475717(-) (comGA) [Bacillus velezensis strain 157]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCAATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment