Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   I3M_RS22930 Genome accession   NZ_CP028116
Coordinates   4061558..4062217 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli O26 str. RM8426     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4056558..4067217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3M_RS22905 (I3M_22885) plsC 4056705..4057442 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  I3M_RS22910 (I3M_22890) parC 4057675..4059933 (-) 2259 WP_001281899.1 DNA topoisomerase IV subunit A -
  I3M_RS22920 (I3M_22900) ygiV 4060479..4060961 (-) 483 WP_000183500.1 GyrI-like domain-containing protein -
  I3M_RS22925 (I3M_22905) ygiW 4061014..4061406 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  I3M_RS22930 (I3M_22910) ciaR 4061558..4062217 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  I3M_RS22935 (I3M_22915) qseC 4062214..4063563 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  I3M_RS22940 (I3M_22920) ygiZ 4063609..4063941 (-) 333 WP_000912120.1 DUF2645 family protein -
  I3M_RS22945 (I3M_22925) mdaB 4064260..4064841 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  I3M_RS22950 (I3M_22930) ygiN 4064872..4065186 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  I3M_RS22955 (I3M_22935) parE 4065234..4067126 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=237833 I3M_RS22930 WP_001221502.1 4061558..4062217(+) (ciaR) [Escherichia coli O26 str. RM8426]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=237833 I3M_RS22930 WP_001221502.1 4061558..4062217(+) (ciaR) [Escherichia coli O26 str. RM8426]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment